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Literature summary for 3.2.1.3 extracted from

  • Han, Y.J.; Yu, T.S.
    Characterization of two forms of glucoamylase from traditional Korean Nuruk fungi, Aspergillus coreanus NR 15-1 (2005), J. Microbiol. Biotechnol., 15, 239-246.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I Aspergillus coreanus
DTT slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I Aspergillus coreanus
EDTA slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I Aspergillus coreanus
N-bromosuccinimide complete inhibition at 1 mM of isozyme GA-I and isozyme GA-II Aspergillus coreanus
pyridoxal 5'-phosphate slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I Aspergillus coreanus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.57
-
starch pH 4.0, 60°C, isozyme GA-I Aspergillus coreanus
6.25
-
starch pH 4.0, 65°C, isozyme GA-II Aspergillus coreanus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ activation at 1 mM of isozyme GA-I and isozyme GA-II Aspergillus coreanus
Mn2+ activation at 1 mM of isozyme GA-I and isozyme GA-II Aspergillus coreanus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
isozyme GA-I, gel filtration Aspergillus coreanus
64000
-
1 * 64000, isozyme GA-I, SDS-PAGE, 1 * 91000, isozyme GA-II, SDS-PAGE Aspergillus coreanus
90000
-
isozyme GA-II, gel filtration Aspergillus coreanus
91000
-
1 * 64000, isozyme GA-I, SDS-PAGE, 1 * 91000, isozyme GA-II, SDS-PAGE Aspergillus coreanus

Organism

Organism UniProt Comment Textmining
Aspergillus coreanus
-
traditional Korean Nuruk fungi, two isozymes GA-I and GA-II
-
Aspergillus coreanus NR 15-1
-
traditional Korean Nuruk fungi, two isozymes GA-I and GA-II
-

Purification (Commentary)

Purification (Comment) Organism
two native isozymes GA-I and GA-II, 5.8 and 9.6fold, respectively, to homogeneity Aspergillus coreanus

Source Tissue

Source Tissue Comment Organism Textmining
additional information culture on uncooked solid-state wheat bran medium Aspergillus coreanus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O soluble starch, raw rice starch, and raw wheat starch, the latter is the preferred substrate Aspergillus coreanus starch + beta-D-glucose
-
?
starch + H2O soluble starch, raw rice starch, and raw wheat starch, the latter is the preferred substrate Aspergillus coreanus NR 15-1 starch + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, isozyme GA-I, SDS-PAGE, 1 * 91000, isozyme GA-II, SDS-PAGE Aspergillus coreanus

Synonyms

Synonyms Comment Organism
GA
-
Aspergillus coreanus
glucoamylase
-
Aspergillus coreanus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
isozyme GA-I Aspergillus coreanus
65
-
isozyme GA-II Aspergillus coreanus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
isozyme GA-I and isozyme GA-II Aspergillus coreanus