Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I | Aspergillus coreanus | |
DTT | slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I | Aspergillus coreanus | |
EDTA | slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I | Aspergillus coreanus | |
N-bromosuccinimide | complete inhibition at 1 mM of isozyme GA-I and isozyme GA-II | Aspergillus coreanus | |
pyridoxal 5'-phosphate | slight inhibition of isozyme GA-II at 1 mM, no inhibition of isozyme GA-I | Aspergillus coreanus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.57 | - |
starch | pH 4.0, 60°C, isozyme GA-I | Aspergillus coreanus | |
6.25 | - |
starch | pH 4.0, 65°C, isozyme GA-II | Aspergillus coreanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activation at 1 mM of isozyme GA-I and isozyme GA-II | Aspergillus coreanus | |
Mn2+ | activation at 1 mM of isozyme GA-I and isozyme GA-II | Aspergillus coreanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
isozyme GA-I, gel filtration | Aspergillus coreanus |
64000 | - |
1 * 64000, isozyme GA-I, SDS-PAGE, 1 * 91000, isozyme GA-II, SDS-PAGE | Aspergillus coreanus |
90000 | - |
isozyme GA-II, gel filtration | Aspergillus coreanus |
91000 | - |
1 * 64000, isozyme GA-I, SDS-PAGE, 1 * 91000, isozyme GA-II, SDS-PAGE | Aspergillus coreanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus coreanus | - |
traditional Korean Nuruk fungi, two isozymes GA-I and GA-II | - |
Aspergillus coreanus NR 15-1 | - |
traditional Korean Nuruk fungi, two isozymes GA-I and GA-II | - |
Purification (Comment) | Organism |
---|---|
two native isozymes GA-I and GA-II, 5.8 and 9.6fold, respectively, to homogeneity | Aspergillus coreanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | culture on uncooked solid-state wheat bran medium | Aspergillus coreanus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | soluble starch, raw rice starch, and raw wheat starch, the latter is the preferred substrate | Aspergillus coreanus | starch + beta-D-glucose | - |
? | |
starch + H2O | soluble starch, raw rice starch, and raw wheat starch, the latter is the preferred substrate | Aspergillus coreanus NR 15-1 | starch + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, isozyme GA-I, SDS-PAGE, 1 * 91000, isozyme GA-II, SDS-PAGE | Aspergillus coreanus |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Aspergillus coreanus |
glucoamylase | - |
Aspergillus coreanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
isozyme GA-I | Aspergillus coreanus |
65 | - |
isozyme GA-II | Aspergillus coreanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
isozyme GA-I and isozyme GA-II | Aspergillus coreanus |