Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.3 extracted from

  • Ichikawa, K.; Tonozuka, T.; Uotsu-Tomita, R.; Akeboshi, H.; Nishikawa, A.; Sakano, Y.
    Purification, characterization, and subsite affinities of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylases (2004), Biosci. Biotechnol. Biochem., 68, 413-420.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain MV1184 Thermoactinomyces vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, recombinant enzyme Thermoactinomyces vulgaris
0.101
-
maltose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
0.38
-
amylotriose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
1.03
-
maltotetraose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
3.94
-
maltopentaose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
7.43
-
maltohexaose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
7.59
-
maltoheptaose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris

Organism

Organism UniProt Comment Textmining
Thermoactinomyces vulgaris
-
-
-
Thermoactinomyces vulgaris R-47
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 50°C for 30 min, two steps of ion exchange chromatography, and gel filtration Thermoactinomyces vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
Thermoactinomyces vulgaris maltohexaose + D-glucose
-
?
maltoheptaose + H2O
-
Thermoactinomyces vulgaris R-47 maltohexaose + D-glucose
-
?
maltononaose + H2O
-
Thermoactinomyces vulgaris maltooctaose + beta-D-glucose
-
?
maltooctaose + H2O
-
Thermoactinomyces vulgaris maltoheptaose + beta-D-glucose
-
?
maltopentadecaose + H2O low activity Thermoactinomyces vulgaris maltotetradecaose + beta-D-glucose
-
?
maltopentaose + H2O preferred substrate Thermoactinomyces vulgaris maltotetraose + beta-D-glucose
-
?
maltopentaose + H2O preferred substrate Thermoactinomyces vulgaris R-47 maltotetraose + beta-D-glucose
-
?
maltose + H2O
-
Thermoactinomyces vulgaris beta-D-glucose + D-glucose
-
?
maltose + H2O
-
Thermoactinomyces vulgaris R-47 beta-D-glucose + D-glucose
-
?
maltotetraose + H2O preferred substrate Thermoactinomyces vulgaris maltotriose + beta-D-glucose
-
?
maltotetraose + H2O preferred substrate Thermoactinomyces vulgaris R-47 maltotriose + beta-D-glucose
-
?
maltotriose + H2O
-
Thermoactinomyces vulgaris maltose + beta-D-glucose
-
?
additional information no activity with pullulan, dextran, and isomaltose Thermoactinomyces vulgaris ?
-
?
additional information no activity with pullulan, dextran, and isomaltose Thermoactinomyces vulgaris R-47 ?
-
?
starch + H2O soluble starch, very low activity Thermoactinomyces vulgaris starch + beta-D-glucose
-
?

Synonyms

Synonyms Comment Organism
GA
-
Thermoactinomyces vulgaris
glucoamylase
-
Thermoactinomyces vulgaris
maltooligosaccharide-metabolizing enzyme
-
Thermoactinomyces vulgaris
tGA
-
Thermoactinomyces vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermoactinomyces vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable up to Thermoactinomyces vulgaris
68
-
4.5% remaining activity Thermoactinomyces vulgaris
70
-
inactivation Thermoactinomyces vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
maltotetraose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
16.9
-
maltose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
55
-
maltohexaose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
56.3
-
maltopentaose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
61.6
-
maltoheptaose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
70.7
-
maltotetraose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris
76.4
-
maltotriose pH 6.5, 40°C, recombinant enzyme Thermoactinomyces vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Thermoactinomyces vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 stable, recombinant enzyme Thermoactinomyces vulgaris