Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain MV1184 | Thermoactinomyces vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, recombinant enzyme | Thermoactinomyces vulgaris | |
0.101 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
0.38 | - |
amylotriose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
1.03 | - |
maltotetraose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
3.94 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
7.43 | - |
maltohexaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
7.59 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces vulgaris | - |
- |
- |
Thermoactinomyces vulgaris R-47 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 50°C for 30 min, two steps of ion exchange chromatography, and gel filtration | Thermoactinomyces vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + H2O | - |
Thermoactinomyces vulgaris | maltohexaose + D-glucose | - |
? | |
maltoheptaose + H2O | - |
Thermoactinomyces vulgaris R-47 | maltohexaose + D-glucose | - |
? | |
maltononaose + H2O | - |
Thermoactinomyces vulgaris | maltooctaose + beta-D-glucose | - |
? | |
maltooctaose + H2O | - |
Thermoactinomyces vulgaris | maltoheptaose + beta-D-glucose | - |
? | |
maltopentadecaose + H2O | low activity | Thermoactinomyces vulgaris | maltotetradecaose + beta-D-glucose | - |
? | |
maltopentaose + H2O | preferred substrate | Thermoactinomyces vulgaris | maltotetraose + beta-D-glucose | - |
? | |
maltopentaose + H2O | preferred substrate | Thermoactinomyces vulgaris R-47 | maltotetraose + beta-D-glucose | - |
? | |
maltose + H2O | - |
Thermoactinomyces vulgaris | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Thermoactinomyces vulgaris R-47 | beta-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | preferred substrate | Thermoactinomyces vulgaris | maltotriose + beta-D-glucose | - |
? | |
maltotetraose + H2O | preferred substrate | Thermoactinomyces vulgaris R-47 | maltotriose + beta-D-glucose | - |
? | |
maltotriose + H2O | - |
Thermoactinomyces vulgaris | maltose + beta-D-glucose | - |
? | |
additional information | no activity with pullulan, dextran, and isomaltose | Thermoactinomyces vulgaris | ? | - |
? | |
additional information | no activity with pullulan, dextran, and isomaltose | Thermoactinomyces vulgaris R-47 | ? | - |
? | |
starch + H2O | soluble starch, very low activity | Thermoactinomyces vulgaris | starch + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Thermoactinomyces vulgaris |
glucoamylase | - |
Thermoactinomyces vulgaris |
maltooligosaccharide-metabolizing enzyme | - |
Thermoactinomyces vulgaris |
tGA | - |
Thermoactinomyces vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermoactinomyces vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to | Thermoactinomyces vulgaris |
68 | - |
4.5% remaining activity | Thermoactinomyces vulgaris |
70 | - |
inactivation | Thermoactinomyces vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
maltotetraose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
16.9 | - |
maltose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
55 | - |
maltohexaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
56.3 | - |
maltopentaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
61.6 | - |
maltoheptaose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
70.7 | - |
maltotetraose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris | |
76.4 | - |
maltotriose | pH 6.5, 40°C, recombinant enzyme | Thermoactinomyces vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Thermoactinomyces vulgaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | stable, recombinant enzyme | Thermoactinomyces vulgaris |