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Literature summary for 3.2.1.3 extracted from

  • Chou, W.I.; Pai, T.W.; Liu, S.H.; Hsiung, B.K.; Chang, M.D.
    The family 21 carbohydrate-binding module of glucoamylase from Rhizopus oryzae consists of two sites playing distinct roles in ligand binding (2006), Biochem. J., 396, 469-477.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.3

Application

Application Comment Organism
industry the enzyme is useful in industrial applications as glycoside hydrolase Rhizopus arrhizus

Protein Variants

Protein Variants Comment Organism
W47A site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme Rhizopus arrhizus
Y32A site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme Rhizopus arrhizus
Y32A/Y47A site-directed mutagenesis, the mutant shows altered kinetics and starch binding compared to the wild-type enzyme Rhizopus arrhizus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information ligand binding kinetics, wild-type enzyme and mutants Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Rhizopus arrhizus
-
 starch + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose structure-function relationship, ligand binding Rhizopus arrhizus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O
-
 Rhizopus arrhizus alpha-cyclodextrin + beta-D-glucose
-
 ?
beta-cyclodextrin + H2O
-
 Rhizopus arrhizus beta-cyclodextrin + beta-D-glucose
-
 ?
gamma-cyclodextrin + H2O
-
 Rhizopus arrhizus gamma-cyclodextrin + beta-D-glucose
-
 ?
isomaltotriose + H2O
-
 Rhizopus arrhizus ? + beta-D-glucose
-
 ?
maltoheptaose + H2O
-
 Rhizopus arrhizus maltohexaose + D-glucose
-
 ?
maltohexaose + H2O
-
 Rhizopus arrhizus maltopentaose + D-glucose
-
 ?
maltopentaose + H2O
-
 Rhizopus arrhizus maltotetraose + beta-D-glucose
-
 ?
maltotriose + H2O
-
 Rhizopus arrhizus maltose + beta-D-glucose
-
 ?
starch + H2O
-
 Rhizopus arrhizus starch + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
More secondary structure analysis, the enzyme contains a family 21 carbohydrate-binding module involving residues W47, Y83, and Y93, the enzyme consists of a catalytic domain and a starch binding domain connected by an O-glycosylated peptide linker located at the N-terminus, molecular modelling, structure-function relatioship Rhizopus arrhizus

Synonyms

Synonyms Comment Organism
GA
-
 Rhizopus arrhizus
glucoamylase
-
 Rhizopus arrhizus
More the enzyme belongs to the glycohydrolase family 21 Rhizopus arrhizus