Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme is potentially useful in improvement of industrial starch processing by eliminating the need to adjust both pH and temperature | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
gene ssg, phylogenetic analysis, expression in Escherichia coli | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97ZD0 | gene ssg | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 80.9fold from Escherichia coli by heat treatment at 70°C, hydrophobic interaction chromatography, and gel filtration | Saccharolobus solfataricus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose | reaction mechanism depends on the substrate, i.e. hydrolysis or transglycosylation, overview | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
56.3 | - |
purified recombinant enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltooligomers + H2O | substrate specificity | Saccharolobus solfataricus | ? + beta-D-glucose | - |
r | |
maltotriose + H2O | exoglycosidic hydrolysis of terminal glucose in forward reaction, preferred substrate, synthesis of isomaltose from beta-D-glucose in the reverse reaction with low activity | Saccharolobus solfataricus | maltose + beta-D-glucose | anomeric product configuration analysis at 80°C | r | |
additional information | bifunctional enzyme performing hydrolysis or transglycosylation depending on the substrate | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Saccharolobus solfataricus |
SSG | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 90% of maximal activity at 80°C, low activity at 100°C | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | - |
Saccharolobus solfataricus |