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Literature summary for 3.2.1.3 extracted from

  • Nielsen, B.R.; Lehmbeck, J.; Frandsen, T.P.
    Cloning, heterologous expression, and enzymatic characterization of a thermostable glucoamylase from Talaromyces emersonii (2002), Protein Expr. Purif., 26, 1-8.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus niger Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
maltoheptaose 45°C, pH 4.5 Rasamsonia emersonii
3.8
-
maltose 45°C, pH 4.5 Rasamsonia emersonii
53.6
-
isomaltose 45°C, pH 4.5 Rasamsonia emersonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62800
-
x * 62800, deduced from nucleotide sequence Rasamsonia emersonii
70000
-
x * 70000, SDS-PAGE, recombinant glucoamylase Rasamsonia emersonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Rasamsonia emersonii
-
beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii Q9C1V4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Rasamsonia emersonii

Purification (Commentary)

Purification (Comment) Organism
recombinant glucoamylase, ultrafiltration, SP-Sepharose, Q-Sepharose Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O
-
Rasamsonia emersonii beta-D-glucose
-
r
maltoheptaose + H2O
-
Rasamsonia emersonii maltohexaose + D-glucose
-
?
maltose + H2O
-
Rasamsonia emersonii beta-D-glucose + D-glucose
-
r
starch + H2O
-
Rasamsonia emersonii beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
? x * 62800, deduced from nucleotide sequence Rasamsonia emersonii
? x * 70000, SDS-PAGE, recombinant glucoamylase Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
GA
-
Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Rasamsonia emersonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90 approx. 15% of maximal activity at 25°C, approx. 20% of maximal activity a 90°C Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
approx. 20% and 50% losss of activity after 20 h and 48 h, respectively, in the presence of 30% glucose Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7
-
isomaltose 45°C, pH 4.5 Rasamsonia emersonii
31.3
-
maltose 45°C, pH 4.5 Rasamsonia emersonii
221.3
-
maltoheptaose 45°C, pH 4.5 Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Rasamsonia emersonii

pH Range

pH Minimum pH Maximum Comment Organism
3 7 approx. 50% of maximal activity at pH 3.0, almost no activity at pH 7.0 Rasamsonia emersonii

pI Value

Organism Comment pI Value Maximum pI Value
Rasamsonia emersonii
-
-
3.5