Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris, Saccharomyces cerevisiae and Aspergillus niger | Aspergillus awamori |
Crystallization (Comment) | Organism |
---|---|
- |
Saccharomycopsis fibuligera |
crystal structure of glucoamylase at 2.2-2.4 A resolution | Aspergillus awamori |
Protein Variants | Comment | Organism |
---|---|---|
E180Q | mutant of G1, 48% of wild-type kcat for maltose, 88% of kcat for maltotriose | Aspergillus niger |
E400C | further oxidation of Cys thiol group to sulfinic acid, up to 300% higher kcat and decreased Km compared to wild-type, depending on substrate | Aspergillus awamori |
W120F | mutant of G1, 3% of wild-type kcat for maltose, 2% of kcat for maltotriose | Aspergillus niger |
W317F | mutant of G1, 90% of wild-type kcat for maltose, 97% of kcat for maltotriose | Aspergillus niger |
W52F | mutant of G2, almost no activity with maltose and maltotriose | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | - |
Aspergillus awamori | |
acarbose | - |
Aspergillus awamori | |
beta-O-acarviosine | - |
Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
maltotetraose | 8°C, pH 4.5, wild-type G1 | Aspergillus niger | |
0.058 | - |
maltotetraose | 8°C, pH 4.5, wild-type G2 | Aspergillus niger | |
0.1 | - |
maltopentaose | recombinant glucoamylase | Aspergillus awamori | |
0.11 | - |
maltohexaose | recombinant glucoamylase | Aspergillus awamori | |
0.12 | - |
maltoheptaose | recombinant glucoamylase | Aspergillus awamori | |
0.18 | - |
maltose | 8°C, pH 4.5, wild-type G1 | Aspergillus niger | |
0.18 | - |
maltotetraose | recombinant glucoamylase | Aspergillus awamori | |
0.28 | - |
maltose | 8°C, pH 4.5, wild-type G2 | Aspergillus niger | |
0.29 | - |
maltotriose | recombinant glucoamylase | Aspergillus awamori | |
3 | - |
maltose | recombinant glucoamylase | Aspergillus awamori | |
15 | - |
isomaltotetraose | recombinant glucoamylase | Aspergillus awamori | |
16 | - |
isomaltopentaose | recombinant glucoamylase | Aspergillus awamori | |
17 | - |
isomaltoheptaose | recombinant glucoamylase | Aspergillus awamori | |
18 | - |
isomaltotriose | recombinant glucoamylase | Aspergillus awamori | |
18 | - |
isomaltohexaose | recombinant glucoamylase | Aspergillus awamori | |
38 | - |
isomaltose | recombinant glucoamylase | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82327 | - |
x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris | Aspergillus awamori |
82839 | - |
x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger | Aspergillus awamori |
83869 | - |
x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae | Aspergillus awamori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Aspergillus niger | - |
beta-D-glucose + ? | - |
? | |
starch + H2O | Aspergillus awamori | - |
beta-D-glucose + ? | - |
? | |
starch + H2O | Saccharomycopsis fibuligera | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus niger | - |
- |
- |
Saccharomycopsis fibuligera | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltoheptaose + H2O | - |
Aspergillus awamori | beta-D-glucose | - |
? | |
isomaltohexaose + H2O | - |
Aspergillus awamori | beta-D-glucose | - |
? | |
isomaltopentaose + H2O | - |
Aspergillus awamori | beta-D-glucose | - |
? | |
isomaltose + H2O | - |
Aspergillus awamori | beta-D-glucose | - |
? | |
isomaltotetraose + H2O | - |
Aspergillus awamori | beta-D-glucose | - |
? | |
isomaltotriose + H2O | - |
Aspergillus awamori | beta-D-glucose | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | maltohexaose + D-glucose | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori | maltopentaose + D-glucose | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori | maltotetraose + beta-D-glucose | - |
? | |
maltose + H2O | - |
Aspergillus niger | beta-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Aspergillus awamori | beta-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | - |
Aspergillus niger | ? | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori | maltotriose + beta-D-glucose | - |
? | |
maltotriose + 2 H2O | - |
Aspergillus awamori | 3 beta-D-glucose | - |
? | |
starch + H2O | - |
Aspergillus niger | beta-D-glucose + ? | - |
? | |
starch + H2O | - |
Aspergillus awamori | beta-D-glucose + ? | - |
? | |
starch + H2O | - |
Saccharomycopsis fibuligera | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris | Aspergillus awamori |
? | x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger | Aspergillus awamori |
? | x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae | Aspergillus awamori |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Aspergillus niger |
GA | - |
Aspergillus awamori |
GA | - |
Saccharomycopsis fibuligera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
isomaltose | recombinant glucoamylase | Aspergillus awamori | |
0.33 | - |
maltose | 8°C, pH 4.5, wild-type G1 | Aspergillus niger | |
0.46 | - |
maltose | 8°C, pH 4.5, wild-type G2 | Aspergillus niger | |
0.64 | - |
isomaltotriose | recombinant glucoamylase | Aspergillus awamori | |
0.99 | - |
isomaltotetraose | recombinant glucoamylase | Aspergillus awamori | |
1.2 | - |
isomaltopentaose | recombinant glucoamylase | Aspergillus awamori | |
1.3 | - |
isomaltoheptaose | recombinant glucoamylase | Aspergillus awamori | |
1.5 | - |
isomaltohexaose | recombinant glucoamylase | Aspergillus awamori | |
2 | 8 | maltotriose | recombinant glucoamylase | Aspergillus awamori | |
3.2 | - |
maltotetraose | 8°C, pH 4.5, wild-type G1 and G2 | Aspergillus niger | |
6.8 | - |
maltose | recombinant glucoamylase | Aspergillus awamori | |
44 | - |
maltopentaose | recombinant glucoamylase | Aspergillus awamori | |
45 | - |
maltotetraose | recombinant glucoamylase | Aspergillus awamori | |
49 | - |
maltohexaose | recombinant glucoamylase | Aspergillus awamori | |
54 | - |
maltoheptaose | recombinant glucoamylase | Aspergillus awamori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000001 | - |
acarbose | - |
Aspergillus awamori | |
0.000098 | - |
beta-O-acarviosine | - |
Aspergillus awamori | |
0.046 | - |
1-deoxynojirimycin | - |
Aspergillus awamori |