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Literature summary for 3.2.1.3 extracted from

  • Sauer, J.; Sigurskjold, B.W.; Christensen, U.; Frandsen, T.P.; Mirgorodskaya, E.; Harrison, M.; Roepstorff, P.; Svensson, B.
    Glucoamylase: structure/function relationships, and protein engineering (2000), Biochim. Biophys. Acta, 1543, 275-293.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris, Saccharomyces cerevisiae and Aspergillus niger Aspergillus awamori

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Saccharomycopsis fibuligera
crystal structure of glucoamylase at 2.2-2.4 A resolution Aspergillus awamori

Protein Variants

Protein Variants Comment Organism
E180Q mutant of G1, 48% of wild-type kcat for maltose, 88% of kcat for maltotriose Aspergillus niger
E400C further oxidation of Cys thiol group to sulfinic acid, up to 300% higher kcat and decreased Km compared to wild-type, depending on substrate Aspergillus awamori
W120F mutant of G1, 3% of wild-type kcat for maltose, 2% of kcat for maltotriose Aspergillus niger
W317F mutant of G1, 90% of wild-type kcat for maltose, 97% of kcat for maltotriose Aspergillus niger
W52F mutant of G2, almost no activity with maltose and maltotriose Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin
-
Aspergillus awamori
acarbose
-
Aspergillus awamori
beta-O-acarviosine
-
Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
maltotetraose 8°C, pH 4.5, wild-type G1 Aspergillus niger
0.058
-
maltotetraose 8°C, pH 4.5, wild-type G2 Aspergillus niger
0.1
-
maltopentaose recombinant glucoamylase Aspergillus awamori
0.11
-
maltohexaose recombinant glucoamylase Aspergillus awamori
0.12
-
maltoheptaose recombinant glucoamylase Aspergillus awamori
0.18
-
maltose 8°C, pH 4.5, wild-type G1 Aspergillus niger
0.18
-
maltotetraose recombinant glucoamylase Aspergillus awamori
0.28
-
maltose 8°C, pH 4.5, wild-type G2 Aspergillus niger
0.29
-
maltotriose recombinant glucoamylase Aspergillus awamori
3
-
maltose recombinant glucoamylase Aspergillus awamori
15
-
isomaltotetraose recombinant glucoamylase Aspergillus awamori
16
-
isomaltopentaose recombinant glucoamylase Aspergillus awamori
17
-
isomaltoheptaose recombinant glucoamylase Aspergillus awamori
18
-
isomaltotriose recombinant glucoamylase Aspergillus awamori
18
-
isomaltohexaose recombinant glucoamylase Aspergillus awamori
38
-
isomaltose recombinant glucoamylase Aspergillus awamori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82327
-
x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris Aspergillus awamori
82839
-
x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger Aspergillus awamori
83869
-
x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae Aspergillus awamori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Aspergillus niger
-
beta-D-glucose + ?
-
?
starch + H2O Aspergillus awamori
-
beta-D-glucose + ?
-
?
starch + H2O Saccharomycopsis fibuligera
-
beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus niger
-
-
-
Saccharomycopsis fibuligera
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltoheptaose + H2O
-
Aspergillus awamori beta-D-glucose
-
?
isomaltohexaose + H2O
-
Aspergillus awamori beta-D-glucose
-
?
isomaltopentaose + H2O
-
Aspergillus awamori beta-D-glucose
-
?
isomaltose + H2O
-
Aspergillus awamori beta-D-glucose
-
?
isomaltotetraose + H2O
-
Aspergillus awamori beta-D-glucose
-
?
isomaltotriose + H2O
-
Aspergillus awamori beta-D-glucose
-
?
maltoheptaose + H2O
-
Aspergillus awamori maltohexaose + D-glucose
-
?
maltohexaose + H2O
-
Aspergillus awamori maltopentaose + D-glucose
-
?
maltopentaose + H2O
-
Aspergillus awamori maltotetraose + beta-D-glucose
-
?
maltose + H2O
-
Aspergillus niger beta-D-glucose + D-glucose
-
?
maltose + H2O
-
Aspergillus awamori beta-D-glucose + D-glucose
-
?
maltotetraose + H2O
-
Aspergillus niger ?
-
?
maltotetraose + H2O
-
Aspergillus awamori maltotriose + beta-D-glucose
-
?
maltotriose + 2 H2O
-
Aspergillus awamori 3 beta-D-glucose
-
?
starch + H2O
-
Aspergillus niger beta-D-glucose + ?
-
?
starch + H2O
-
Aspergillus awamori beta-D-glucose + ?
-
?
starch + H2O
-
Saccharomycopsis fibuligera beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
? x * 82327, mass spectroscopy, recombinant GA expressed in Pichia pastoris Aspergillus awamori
? x * 82839, mass spectroscopy, recombinant GA expressed in Aspergillus niger Aspergillus awamori
? x * 83869, mass spectroscopy, recombinant GA expressed in Saccharomyces cerevisiae Aspergillus awamori

Synonyms

Synonyms Comment Organism
GA
-
Aspergillus niger
GA
-
Aspergillus awamori
GA
-
Saccharomycopsis fibuligera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
isomaltose recombinant glucoamylase Aspergillus awamori
0.33
-
maltose 8°C, pH 4.5, wild-type G1 Aspergillus niger
0.46
-
maltose 8°C, pH 4.5, wild-type G2 Aspergillus niger
0.64
-
isomaltotriose recombinant glucoamylase Aspergillus awamori
0.99
-
isomaltotetraose recombinant glucoamylase Aspergillus awamori
1.2
-
isomaltopentaose recombinant glucoamylase Aspergillus awamori
1.3
-
isomaltoheptaose recombinant glucoamylase Aspergillus awamori
1.5
-
isomaltohexaose recombinant glucoamylase Aspergillus awamori
2 8 maltotriose recombinant glucoamylase Aspergillus awamori
3.2
-
maltotetraose 8°C, pH 4.5, wild-type G1 and G2 Aspergillus niger
6.8
-
maltose recombinant glucoamylase Aspergillus awamori
44
-
maltopentaose recombinant glucoamylase Aspergillus awamori
45
-
maltotetraose recombinant glucoamylase Aspergillus awamori
49
-
maltohexaose recombinant glucoamylase Aspergillus awamori
54
-
maltoheptaose recombinant glucoamylase Aspergillus awamori

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000001
-
acarbose
-
Aspergillus awamori
0.000098
-
beta-O-acarviosine
-
Aspergillus awamori
0.046
-
1-deoxynojirimycin
-
Aspergillus awamori