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Literature summary for 3.2.1.3 extracted from

  • Fierobe, H.P.; Mirgorodskaya, E.; Frandsen, T.P.; Roepstorff, P.; Svensson, B.
    Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts (1997), Protein Expr. Purif., 9, 159-170.
    View publication on PubMed
Search for more literature for 3.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris, Saccharomyces cerevisiae or Aspergillus niger. In Pichia pastoris the C320A/E400C double mutant is produced at 75% of the wild-type glucoamylase, while the corresponding single mutants are produced at 1% and 20% of the wild-type level, respectively Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 maltotriose recombinant enzyme expressed in Saccharomyces cerevisiae Aspergillus awamori
0.28
-
 maltotriose recombinant enzyme expressed in Aspergillus niger Aspergillus awamori
0.29
-
 maltotriose recombinant enzyme expressed in Pichia pastoris Aspergillus awamori
1.21
-
 maltose recombinant enzyme expressed in Aspergillus niger Aspergillus awamori
1.82
-
 maltose recombinant enzyme expressed in Saccharomyces cerevisiae Aspergillus awamori
2.97
-
 maltose recombinant enzyme expressed in Pichia pastoris Aspergillus awamori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80370
-
 MALDI-MS Aspergillus awamori
82330
-
 recombinant enzyme expressed in Pichia pastoris, matrix-assisted laser desorption ionization mass spectrometry Aspergillus awamori
82840
-
 recombinant enzyme expressed in Aspergillus niger, MALDI-MS Aspergillus awamori
83870
-
 recombinant enzyme expressed in Saccharomyces cerevisiae, matrix-assisted laser desorption ionization mass spectrometry Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
 wild-type and mutant enzyme C320A/E400C
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O
-
 Aspergillus awamori glucose
-
 ?
maltoheptaose + H2O
-
 Aspergillus awamori ?
-
 ?
maltohexaose + H2O
-
 Aspergillus awamori ?
-
 ?
maltopentaose + H2O
-
 Aspergillus awamori ?
-
 ?
maltose + H2O
-
 Aspergillus awamori 2 glucose
-
 ?
maltotetraose + H2O
-
 Aspergillus awamori ?
-
 ?
maltotriose + H2O
-
 Aspergillus awamori maltose + glucose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover numbers for the recombinant enzymes with maltose through maltoheptaose as substrates Aspergillus awamori
6.8
-
 maltose recombinant enzyme expressed in Pichia pastoris Aspergillus awamori
10.7
-
 maltose recombinant enzyme expressed in Aspergillus niger Aspergillus awamori
11.3
-
 maltose recombinant enzyme expressed in Saccharomyces cerevisiae Aspergillus awamori
27.6
-
 maltotriose recombinant enzyme expressed in Pichia pastoris Aspergillus awamori
30.9
-
 maltotriose recombinant enzyme expressed in Saccharomyces cerevisiae Aspergillus awamori
33.5
-
 maltotriose recombinant enzyme expressed in Aspergillus niger Aspergillus awamori
44.7
-
 maltotetraose recombinant enzyme expressed in Pichia pastoris Aspergillus awamori