KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.48 mg/ml for starch with glucoamylase II and III. 0.5 mg/ml for starch with glucoamylase I | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
glucoamylase III, gel filtration | Aspergillus oryzae |
67000 | - |
glucoamylase II, gel filtration | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
three enzyme forms: I, II, and III | Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | solid culture on steamed rice | Aspergillus oryzae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Aspergillus oryzae | D-glucose + ? | - |
? | |
amylose + H2O | - |
Aspergillus oryzae | glucose + ? | - |
? | |
beta-cyclodextrin + H2O | no activity | Aspergillus oryzae | ? | - |
? | |
dextran + H2O | no activity | Aspergillus oryzae | ? | - |
? | |
dextrin + H2O | beta-limit dextrin | Aspergillus oryzae | ? | - |
? | |
maltose + H2O | - |
Aspergillus oryzae | 2 glucose | - |
? | |
maltotriose + H2O | - |
Aspergillus oryzae | maltose + glucose | - |
? | |
methyl-alpha-D-glucoside + H2O | no activity | Aspergillus oryzae | methanol + alpha-D-glucose | - |
? | |
pullulan + H2O | no activity | Aspergillus oryzae | ? | - |
? | |
starch + H2O | soluble | Aspergillus oryzae | glucose + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
glucoamylase II and III | Aspergillus oryzae |
60 | - |
glucoamylase I | Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
complete loss of activity above | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | 40°C, 30 min, stable | Aspergillus oryzae |