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Literature summary for 3.2.1.3 extracted from

  • Mitsue, T.; Saha, B.C.; Ueda, S.
    Glucoamylase of Aspergillus oryzae cultured on steamed rice (1979), J. Appl. Biochem., 1, 410-422.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 0.48 mg/ml for starch with glucoamylase II and III. 0.5 mg/ml for starch with glucoamylase I Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
glucoamylase III, gel filtration Aspergillus oryzae
67000
-
glucoamylase II, gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
three enzyme forms: I, II, and III Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture solid culture on steamed rice Aspergillus oryzae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Aspergillus oryzae D-glucose + ?
-
?
amylose + H2O
-
Aspergillus oryzae glucose + ?
-
?
beta-cyclodextrin + H2O no activity Aspergillus oryzae ?
-
?
dextran + H2O no activity Aspergillus oryzae ?
-
?
dextrin + H2O beta-limit dextrin Aspergillus oryzae ?
-
?
maltose + H2O
-
Aspergillus oryzae 2 glucose
-
?
maltotriose + H2O
-
Aspergillus oryzae maltose + glucose
-
?
methyl-alpha-D-glucoside + H2O no activity Aspergillus oryzae methanol + alpha-D-glucose
-
?
pullulan + H2O no activity Aspergillus oryzae ?
-
?
starch + H2O soluble Aspergillus oryzae glucose + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
glucoamylase II and III Aspergillus oryzae
60
-
glucoamylase I Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
complete loss of activity above Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 40°C, 30 min, stable Aspergillus oryzae