General Stability | Organism |
---|---|
5 M guanidine hydrochloride, almost complete denaturation | Aspergillus phoenicis |
8 M urea, partial denaturation | Aspergillus phoenicis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, 48% inhibition, glucoamylase M2 | Aspergillus phoenicis | |
Mn2+ | 1 mM, 12% inhibition, glucoamylase M2 | Aspergillus phoenicis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.92% for soluble starch, glucoamylase M2 | Aspergillus phoenicis | |
0.146 | - |
amylose | glucoamylase M2 | Aspergillus phoenicis | |
0.73 | - |
Dextrin | glucoamylase M2 | Aspergillus phoenicis | |
1.25 | - |
glycogen | glucoamylase M2 | Aspergillus phoenicis | |
1.4 | - |
maltotriose | glucoamylase M2 | Aspergillus phoenicis | |
1.65 | - |
amylopectin | glucoamylase M2 | Aspergillus phoenicis | |
3 | - |
maltose | glucoamylase M2 | Aspergillus phoenicis | |
5.6 | - |
p-nitrophenyl-alpha-D-glucoside | glucoamylase M2 | Aspergillus phoenicis | |
10 | - |
Phenyl-alpha-maltoside | glucoamylase M2 | Aspergillus phoenicis | |
36.4 | - |
isomaltose | glucoamylase M2 | Aspergillus phoenicis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, glucoamylase M2, SDS-PAGE | Aspergillus phoenicis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus phoenicis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 0.55% glucosamine and 12% neutral sugar which consists of a large amount of mannose and small amounts of galactose and glucose | Aspergillus phoenicis |
Purification (Comment) | Organism |
---|---|
glucoamylase M2 | Aspergillus phoenicis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.6 | - |
glucoamylase M1 | Aspergillus phoenicis |
40.8 | - |
glucoamylase M2 | Aspergillus phoenicis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucoside + H2O | - |
Aspergillus phoenicis | 4-nitrophenol + D-glucose | - |
? | |
amylopectin + H2O | - |
Aspergillus phoenicis | D-glucose + ? | - |
? | |
amylose + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
dextrin + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
glycogen + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
isomaltose + H2O | - |
Aspergillus phoenicis | glucose | - |
? | |
maltose + H2O | - |
Aspergillus phoenicis | 2 glucose | - |
? | |
maltotriose + H2O | - |
Aspergillus phoenicis | maltose + glucose | - |
? | |
phenyl alpha-maltoside + H2O | - |
Aspergillus phoenicis | glucose + phenyl alpha-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, glucoamylase M2, SDS-PAGE | Aspergillus phoenicis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 6.0, 5 min, stable up to | Aspergillus phoenicis |
70 | - |
pH 6.0, 5 min, complete loss of activity | Aspergillus phoenicis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
glucoamylase M2 | Aspergillus phoenicis |
4.5 | - |
hydrolysis of starch | Aspergillus phoenicis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
37°C, 1 h, complete loss of activity below | Aspergillus phoenicis |
2.5 | 7.5 | 37°C, 1 h, stable | Aspergillus phoenicis |
9 | - |
37°C, 1 h, complete loss of activity above | Aspergillus phoenicis |