Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | stimulates at 1 mM or at 10 mM | Aspergillus niger | |
iodoacetamide | stimulates | Aspergillus niger | |
Schardinger dextrin | slight stimulation of activity with maltose as substrate | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Aspergillus niger | |
Cu2+ | - |
Aspergillus niger | |
gentiobiose | 20 mM, uncompetitive inhibition with starch as substrate | Aspergillus niger | |
maltitol | 20 mM, noncompetitive inhibition with starch as substrate | Aspergillus niger | |
methyl alpha-D-glucoside | competitive with maltose and non-competitive with starch | Aspergillus niger | |
Ni2+ | - |
Aspergillus niger | |
p-hydroxymercuribenzoate | - |
Aspergillus niger | |
Schardinger dextrin | mixed inhibition with starch | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.025% for starch | Aspergillus niger | |
1.42 | - |
maltotriose | - |
Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
arsenate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
Ba2+ | 1 mM, some stimulation | Aspergillus niger | |
Borate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
Carbonate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
diphosphate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
phosphate | stimulates at 10 mM, 1 mM and 0.1 mM | Aspergillus niger | |
Sn2+ | 1 mM, some stimulation | Aspergillus niger | |
Zn2+ | 1 mM, some stimulation | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | 31% of the activity with starch | Aspergillus niger | glucose + ? | - |
? | |
maltose + H2O | - |
Aspergillus niger | 2 glucose | - |
? | |
maltotriose + H2O | 68% of the activity with starch | Aspergillus niger | maltose + glucose | - |
? | |
panose + H2O | 14% of the activity with starch | Aspergillus niger | ? | - |
? | |
pullulan + H2O | 1.46% of the activity with starch | Aspergillus niger | ? | - |
? | |
starch + H2O | with concentrated solutions of D-glucose, 10% w/v and 30% w/v, small amounts of isomaltose are produced as the sole reversion product | Aspergillus niger | glucose + ? | - |
? | |
sucrose + H2O | 3.3% of carbohydrate | Aspergillus niger | D-glucose + D-fructose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
starch or glycerol improve the thermal stability | Aspergillus niger |
50 | - |
pH 4.5, 30 min, stable | Aspergillus niger |
60 | - |
pH 4.5, 30 min, 10% loss of activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
with maltose or starch as substrate | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1.5 | 6.5 | pH 1.5: about 70% of maximal activity with starch as substrate, about 60% of the activity with maltose as substrate, pH 6.5: about 15% of maximal activity with maltose as substrate, about 55% of maximal activity with starch as substrate | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
40°C, 30 min, 21% loss of activity | Aspergillus niger |
11 | - |
40°C, 30 min, 50% loss of activity | Aspergillus niger |