Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.3 extracted from

  • Abe, J.I.; Nagano, H.; Hizukuri, S.
    Kinetic and structural properties of the three forms of glucoamylase of Rhizopus delemar (1985), J. Appl. Biochem., 7, 235-247.
No PubMed abstract available
Search for more literature for 3.2.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 maltoheptaose
-
 Aspergillus niger
0.1
-
 maltohexaose
-
 Aspergillus niger
0.1
-
 maltoheptaose enzyme form GII Rhizopus arrhizus
0.11
-
 maltohexaose enzyme form GII Rhizopus arrhizus
0.11
-
 maltoheptaose enzyme form GI Rhizopus arrhizus
0.12
-
 maltopentaose
-
 Aspergillus niger
0.12
-
 maltohexaose enzyme form GI Rhizopus arrhizus
0.14
-
 maltotetraose
-
 Aspergillus niger
0.14
-
 maltopentaose enzyme form GII Rhizopus arrhizus
0.15
-
 maltoheptaose
-
 Aspergillus niger
0.15
-
 maltoheptaose enzyme form GIII Rhizopus arrhizus
0.16
-
 maltopentaose enzyme form GI Rhizopus arrhizus
0.16
-
 maltohexaose enzyme form GIII Rhizopus arrhizus
0.17
-
 maltopentaose enzyme form GIII Rhizopus arrhizus
0.21
-
 maltotetraose enzyme form GII Rhizopus arrhizus
0.23
-
 maltotetraose enzyme form GI Rhizopus arrhizus
0.26
-
 maltotetraose enzyme form GIII Rhizopus arrhizus
0.3
-
 maltotriose
-
 Aspergillus niger
0.3
-
 amylopectin enzyme form GIII Rhizopus arrhizus
0.57
-
 maltotriose enzyme form GII Rhizopus arrhizus
0.59
-
 maltotriose enzyme form GI Rhizopus arrhizus
0.64
-
 maltotriose enzyme form GIII Rhizopus arrhizus
0.8
-
 glycogen glycogen from rabbit liver, enzyme form GIII Rhizopus arrhizus
0.91
-
 maltose
-
 Aspergillus niger
1.8
-
 glycogen glycogen from oyster, enzyme form GIII Rhizopus arrhizus
1.9
-
 maltose enzyme form GI or GII Rhizopus arrhizus
2
-
 maltose enzyme form GIII Rhizopus arrhizus
6.1
-
 amylopectin enzyme form GI Rhizopus arrhizus
7.4
-
 amylopectin enzyme form GII Rhizopus arrhizus
13
-
 panose
-
 Aspergillus niger
13
-
 panose enzyme form GII Rhizopus arrhizus
14
-
 panose enzyme form GI or GIII Rhizopus arrhizus
18
-
 pullulan enzyme form GIII Rhizopus arrhizus
40
-
 isomaltose enzyme form GIII Rhizopus arrhizus
42
-
 isomaltose
-
 Aspergillus niger
47
-
 isomaltose enzyme form GI or GII Rhizopus arrhizus
59
-
 amylopectin
-
 Aspergillus niger
210
-
 glycogen glycogen from oyster, enzyme form GI Rhizopus arrhizus
240
-
 glycogen glycogen from rabbit liver, enzyme form GI Rhizopus arrhizus
250
-
 glycogen glycogen from oyster, enzyme form GII Rhizopus arrhizus
300
-
 glycogen glycogen from rabbit liver, enzyme form GII Rhizopus arrhizus
820
-
 glycogen glycogen from rabbit liver Aspergillus niger
1100
-
 glycogen glycogen from oyster Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, enzyme form GI, SDS-PAGE Rhizopus arrhizus
70000
-
 x * 70000, enzyme form GII, SDS-PAGE Rhizopus arrhizus
78000
-
 x * 78000, enzyme form GIII, SDS-PAGE Rhizopus arrhizus

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Rhizopus arrhizus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus niger
glycoprotein
-
 Rhizopus arrhizus
proteolytic modification
-
 Aspergillus niger
proteolytic modification enzyme form GI and GII are transformed products of enzyme form GIII due to the limited action of proteases and glucosidases. GII is formed from GIII upon the release of a peptide portion of approximately 8000 Da. Enzyme form GI is formed from enzyme form GII and GIII on the removal of both peptide and carbohydrate chains and/or glycopeptide fragments Rhizopus arrhizus

Purification (Commentary)

Purification (Comment) Organism
3 enzyme forms: GI, GII and GIII Rhizopus arrhizus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus niger
additional information
-
-
 Rhizopus arrhizus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Aspergillus niger D-glucose + ?
-
 ?
amylopectin + H2O
-
 Rhizopus arrhizus D-glucose + ?
-
 ?
glycogen + H2O
-
 Aspergillus niger glucose + ?
-
 ?
glycogen + H2O
-
 Rhizopus arrhizus beta-D-glucose + glycogen
-
 ?
isomaltose + H2O
-
 Aspergillus niger glucose
-
 ?
isomaltose + H2O
-
 Rhizopus arrhizus glucose
-
 ?
maltoheptaose + H2O
-
 Aspergillus niger ?
-
 ?
maltoheptaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
maltohexaose + H2O
-
 Aspergillus niger ?
-
 ?
maltohexaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
maltopentaose + H2O
-
 Aspergillus niger ?
-
 ?
maltopentaose + H2O
-
 Rhizopus arrhizus ?
-
 ?
maltose + H2O
-
 Aspergillus niger 2 glucose
-
 ?
maltose + H2O
-
 Rhizopus arrhizus 2 glucose
-
 ?
maltotetraose + H2O
-
 Aspergillus niger ?
-
 ?
maltotetraose + H2O
-
 Rhizopus arrhizus ?
-
 ?
maltotriose + H2O
-
 Aspergillus niger maltose + glucose
-
 ?
maltotriose + H2O
-
 Rhizopus arrhizus maltose + glucose
-
 ?
panose + H2O
-
 Aspergillus niger ?
-
 ?
panose + H2O
-
 Rhizopus arrhizus ?
-
 ?
pullulan + H2O
-
 Rhizopus arrhizus beta-D-glucose + pullulan
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, enzyme form GII, SDS-PAGE Rhizopus arrhizus
? x * 78000, enzyme form GIII, SDS-PAGE Rhizopus arrhizus
? x * 61000, enzyme form GI, SDS-PAGE Rhizopus arrhizus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 4.5, 10 min, quite stable up to Rhizopus arrhizus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8.5 40°C, 30 min, stable Rhizopus arrhizus