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Literature summary for 3.2.1.28 extracted from

  • Kord, M.; Youssef, E.; Ahmed, H.; Qaid, E.
    Purification and characterization of trehalase from seeds of chickpea (Cicer arietinum L.) (2013), Turk. J. Biol., 37, 661-669.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
ATP 10% residual activity at 20 mM Cicer arietinum
Borate 16% residual activity at 20 mM Cicer arietinum
Ca2+ 67% residual activity at 20 mM Cicer arietinum
Cu2+ 14% residual activity at 20 mM Cicer arietinum
EDTA 16% residual activity at 20 mM Cicer arietinum
Fe3+ 31% residual activity at 20 mM Cicer arietinum
K+ 69% residual activity at 20 mM Cicer arietinum
Li+ 12% residual activity at 20 mM Cicer arietinum
malate 37% residual activity at 20 mM Cicer arietinum
mannitol competitive inhibitor, 8% residual activity at 20 mM Cicer arietinum
Na+ 61% residual activity at 20 mM Cicer arietinum
succinate 41% residual activity at 20 mM Cicer arietinum
validamycin A competitive inhibitor, 49% residual activity at 5 nM Cicer arietinum
Zn2+ non-competitive inhibitor, 22% residual activity at 20 mM Cicer arietinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.64
-
alpha,alpha-trehalose at pH 5.5 and 55°C Cicer arietinum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 122% activity at 20 mM Cicer arietinum
Mg2+ 133% activity at 20 mM Cicer arietinum
Mn2+ 210% activity at 20 mM Cicer arietinum
Ni2+ 122% activity at 20 mM Cicer arietinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
6 * 38000, SDS-PAGE Cicer arietinum
223000
-
gel filtration Cicer arietinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Cicer arietinum
-
beta-D-glucose + alpha-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Cicer arietinum
-
cultivar Giza 1
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, CM Sepharose column chromatography, and Sephadex G-200 gel filtration Cicer arietinum

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Cicer arietinum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
crude extract, at pH 5.5 and 55°C Cicer arietinum
117
-
after 232fold purification, at pH 5.5 and 55°C Cicer arietinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
Cicer arietinum beta-D-glucose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 38000, SDS-PAGE Cicer arietinum

Synonyms

Synonyms Comment Organism
trehalase
-
Cicer arietinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Cicer arietinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 65 more than 50% activity between 35 and 65°C Cicer arietinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
Cicer arietinum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000004
-
validamycin A at pH 5.5 and 55°C Cicer arietinum
7
-
Zn2+ at pH 5.5 and 55°C Cicer arietinum
9
-
mannitol at pH 5.5 and 55°C Cicer arietinum