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Literature summary for 3.2.1.28 extracted from

  • Silva, M.C.; Terra, W.R.; Ferreira, C.
    The catalytic and other residues essential for the activity of the midgut trehalase from Spodoptera frugiperda (2010), Insect Biochem. Mol. Biol., 40, 733-741.
    View publication on PubMed
Search for more literature for 3.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
-
 Spodoptera frugiperda

Protein Variants

Protein Variants Comment Organism
D322A at least four orders of magnitude less active than wild type trehalase, with no structural difference between mutant and wild type enzyme discernible by circular dichroism. Mutation causes an increase in the activation energy for trehalose hydrolysis Spodoptera frugiperda
E520A at least four orders of magnitude less active than wild type trehalase, with no structural difference between mutant and wild type enzyme discernible by circular dichroism. Mutation causes an increase in the activation energy for trehalose hydrolysis. In presence of azide, activity increases three fold Spodoptera frugiperda
R169A at least four orders of magnitude less active than wild type trehalase, with no structural difference between mutant and wild type enzyme discernible by circular dichroism. Mutation causes an increase in the activation energy for trehalose hydrolysis Spodoptera frugiperda
R222A at least four orders of magnitude less active than wild type trehalase, with no structural difference between mutant and wild type enzyme discernible by circular dichroism. Mutation causes an increase in the activation energy for trehalose hydrolysis Spodoptera frugiperda
R222A/R287A at least four orders of magnitude less active than wild type trehalase, with no structural difference between mutant and wild type enzyme discernible by circular dichroism. Mutation causes an increase in the activation energy for trehalose hydrolysis Spodoptera frugiperda
R287A at least four orders of magnitude less active than wild type trehalase, with no structural difference between mutant and wild type enzyme discernible by circular dichroism. Mutation causes an increase in the activation energy for trehalose hydrolysis Spodoptera frugiperda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 alpha,alpha-trehalose mutant R222A/R287A, pH 7.0, 30°C Spodoptera frugiperda
0.22
-
 alpha,alpha-trehalose mutant E520A, pH 7.0, 30°C Spodoptera frugiperda
0.22
-
 alpha,alpha-trehalose mutant R287A, pH 7.0, 30°C Spodoptera frugiperda
0.3
-
 alpha,alpha-trehalose wild-type, pH 7.0, 30°C Spodoptera frugiperda
0.45
-
 alpha,alpha-trehalose mutant R169A, pH 7.0, 30°C Spodoptera frugiperda
1
-
 alpha,alpha-trehalose mutant D322A, pH 7.0, 30°C Spodoptera frugiperda
3.33
-
 alpha,alpha-trehalose mutant R222A, pH 7.0, 30°C Spodoptera frugiperda

Organism

Organism UniProt Comment Textmining
Spodoptera frugiperda Q0ZIF5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Spodoptera frugiperda

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
 Spodoptera frugiperda 2 D-glucose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00045
-
 alpha,alpha-trehalose mutant R222A/R287A, pH 7.0, 30°C Spodoptera frugiperda
0.0005
-
 alpha,alpha-trehalose mutant E520A, pH 7.0, 30°C Spodoptera frugiperda
0.0009
-
 alpha,alpha-trehalose mutant R287A, pH 7.0, 30°C Spodoptera frugiperda
0.12
-
 alpha,alpha-trehalose mutant R169A, pH 7.0, 30°C Spodoptera frugiperda
0.18
-
 alpha,alpha-trehalose mutant D322A, pH 7.0, 30°C Spodoptera frugiperda
0.3
-
 alpha,alpha-trehalose mutant R222A, pH 7.0, 30°C Spodoptera frugiperda
730
-
 alpha,alpha-trehalose wild-type, pH 7.0, 30°C Spodoptera frugiperda

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.008
-
 alpha,alpha-trehalose mutant R222A/R287A, pH 7.0, 30°C Spodoptera frugiperda
0.009
-
 alpha,alpha-trehalose mutant R222A, pH 7.0, 30°C Spodoptera frugiperda
0.027
-
 alpha,alpha-trehalose mutant E520A, pH 7.0, 30°C Spodoptera frugiperda
0.045
-
 alpha,alpha-trehalose mutant R287A, pH 7.0, 30°C Spodoptera frugiperda
0.18
-
 alpha,alpha-trehalose mutant D322A, pH 7.0, 30°C Spodoptera frugiperda
0.27
-
 alpha,alpha-trehalose mutant R169A, pH 7.0, 30°C Spodoptera frugiperda
2400
-
 alpha,alpha-trehalose wild-type, pH 7.0, 30°C Spodoptera frugiperda