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Literature summary for 3.2.1.28 extracted from

  • Chen, J.; Tang, B.; Chen, H.; Yao, Q.; Huang, X.; Chen, J.; Zhang, D.; Zhang, W.
    Different functions of the insect soluble and membrane-bound trehalase genes in chitin biosynthesis revealed by RNA interference (2010), PLoS ONE, 5, e10133.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
soluble trehalase gene SeTre-1, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, developmental expression pattern of the two trehalase genes SeTre-1 and SeTre-2, overview Spodoptera exigua

Protein Variants

Protein Variants Comment Organism
additional information knockout by RNAi of genes SeTre-1 and SeTre-2 leads to significant higher mortality rates during the larva-pupa stage and pupa-adult stage, lethal phenotypes, overview. Knockdown of SeTre-1 gene largely inhibited the expression of chitin synthase gene A and reduced the chitin content in the cuticle to two-thirds relative to the control insects Spodoptera exigua

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol soluble-bound isozyme SeTre-2 Spodoptera exigua 5829
-
membrane membrane-bound isozyme SeTre-2 Spodoptera exigua 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66480
-
x * 66480, SeTre-1, sequence calculation Spodoptera exigua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Spodoptera exigua
-
2 D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Spodoptera exigua
-
genes SeTre-1 and SeTre-2
-

Source Tissue

Source Tissue Comment Organism Textmining
cuticle high expression level of isozyme SeTre-1 Spodoptera exigua
-
fat body high expression level of isozyme SeTre-2 Spodoptera exigua
-
larva fifth instar larvae Spodoptera exigua
-
malpighian tubule high expression level of isozyme SeTre-1 Spodoptera exigua
-
additional information tissue distribution of soluble trehalase SeTre-1, developmental expression pattern of the two trehalase genes SeTre-1 and SeTre-2, overview Spodoptera exigua
-
trachea high expression level of isozyme SeTre-2 Spodoptera exigua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
Spodoptera exigua 2 D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 66480, SeTre-1, sequence calculation Spodoptera exigua

Synonyms

Synonyms Comment Organism
alpha-glucoside-1-glucohydrolase
-
Spodoptera exigua
SeTre-1
-
Spodoptera exigua
SeTre-2
-
Spodoptera exigua
trehalase
-
Spodoptera exigua

pI Value

Organism Comment pI Value Maximum pI Value
Spodoptera exigua SeTre-1, sequence calculation
-
4.75

General Information

General Information Comment Organism
metabolism different functions of the insect soluble and membrane-bound trehalase genes in chitin biosynthesis, overview Spodoptera exigua