Literature summary for 3.2.1.28 extracted from

He, S.; Bystricky, K.; Leon, S.; Francois, J.M.; Parrou, J.L.
The Saccharomyces cerevisiae vacuolar acid trehalase is targeted at the cell surface for its physiological function (2009), FEBS J., 276, 5432-5446.

Search for more literature for 3.2.1.28

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GFP-tagged Ath1 N-terminal domain	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	complementation of the Saccharomyces cerevisiae suc2D mutant SEY6210 strain with different Ath1-invertase chimera, where the Suc2 signal peptide is replaced by full-length ATH1 sequence, overview. Hybrid Ath1 truncated mutants fused at their C-terminus with the yeast internal invertase reveal that a 131 amino acid N-terminal fragment of Ath1 is sufficient to target the fusion protein to the cell surface, enabling growth of the suc2DELTA mutant on sucrose. Removal of the N-terminus of Ath1 causes a strict vacuolar localization. Fusion of the signal peptide of invertase to N-terminally truncated Ath1 allows the ath1D mutant to grow on trehalose, whereas the signal sequence of the vacuolar-targeted Pep4 constrained Ath1 in the vacuole and prevents growth of this mutant on trehalose	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell surface	Ath1	Saccharomyces cerevisiae	9986	-
additional information	Ath1 possesses a transmembrane domain. Existence of a secretion sequence in Ath1	Saccharomyces cerevisiae	-	-
vacuole	Ath1	Saccharomyces cerevisiae	5773	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	x * 30000, about, recombinant GFP-tagged Ath1 N-terminal domain	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha,alpha-trehalose + H2O	Saccharomyces cerevisiae	trehalose is one of the major storage carbohydrates in the yeast Saccharomyces cerevisiae	2 D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	neutral Nth1 and Nth2 and acid Ath1, encoded by gene ath1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha,alpha-trehalose + H2O	-	Saccharomyces cerevisiae	2 D-glucose	-	?
alpha,alpha-trehalose + H2O	trehalose is one of the major storage carbohydrates in the yeast Saccharomyces cerevisiae	Saccharomyces cerevisiae	2 D-glucose	-	?
additional information	the 131 amino acid N-terminus of Ath1 are sufficient for invertase secretion, and the short transmembrane domain, located at the N-terminus, is indispensable for Ath1 function, overview	Saccharomyces cerevisiae	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 30000, about, recombinant GFP-tagged Ath1 N-terminal domain	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Ath1	-	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	Ath1	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)