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Literature summary for 3.2.1.28 extracted from
- Purification and properties of two types of soluble trehalases from embryonic larvae of the silkworm, Bombyx mori (2006), J. Insect Biotechnol. Sericol., 75, 1-8.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
trehalose | soluble trehalase P II, inacetate buffer, pH 5.5, at 30°C | Bombyx mori |  |
| 1.56 | - |
trehalose | soluble trehalase P I, in acetate buffer, pH 5.5, at 30°C | Bombyx mori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
soluble trehalase P I, SDS-PAGE | Bombyx mori |
| 140000 | - |
soluble trehalase P II, SDS-PAGE | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S-300 gel filtration, DEAE-cellulofine column chromatography, and Con A-Sepharose column chromatography | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Bombyx mori | - |
| embryo | - |
Bombyx mori | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
soluble trehalase P I from supernatant, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
| 0.01 | - |
soluble trehalase P II from supernatant, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
| 1.03 | - |
soluble trehalase P I after 86.9fold purification, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
| 2.9 | - |
soluble trehalase P II after 245.7fold purification, in acetate buffer, pH 5.5, at 30°C | Bombyx mori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | - |
Bombyx mori | D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid trehalase | - |
Bombyx mori |
| alpha,alpha glucoside 1-glucohydrolase | - |
Bombyx mori |
| soluble P I type trehalase | - |
Bombyx mori |
| soluble P II type trehalase | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | soluble trehalase P I | Bombyx mori |
| 50 | - |
above 50°C, soluble trehalase P I | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
soluble trehalase P II | Bombyx mori |
| 6.5 | - |
soluble trehalase P I | Bombyx mori |
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