Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Apis mellifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
trehalose | recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C | Apis mellifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
wild type enzyme, SDS-PAGE | Apis mellifera |
69180 | - |
calculated from amino acid sequence | Apis mellifera |
76000 | - |
recombinant enzyme, SDS-PAGE | Apis mellifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis mellifera | A8J4S9 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose Fast Flow column chromatography and Sephacryl S-100 gel filtration | Apis mellifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trehalose + H2O | - |
Apis mellifera | D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acid trehalase | - |
Apis mellifera |
alpha,alpha trehalase | - |
Apis mellifera |
alpha,alpha trehalose glucohydrolase | - |
Apis mellifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Apis mellifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme, stable under incubation at 40°C or lower for 15 min | Apis mellifera |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
662 | - |
trehalose | recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C | Apis mellifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10.5 | recombinant enzyme | Apis mellifera |