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Literature summary for 3.2.1.28 extracted from

  • Lee, J.H.; Saito, S.; Mori, H.; Nishimoto, M.; Okuyama, M.; Kim, D.; Wongchawalit, J.; Kimura, A.; Chiba, S.
    Molecular cloning of cDNA for trehalase from the European honeybee, Apis mellifera L., and its heterologous expression in Pichia pastoris (2007), Biosci. Biotechnol. Biochem., 71, 2256-2265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Apis mellifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
trehalose recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C Apis mellifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
wild type enzyme, SDS-PAGE Apis mellifera
69180
-
calculated from amino acid sequence Apis mellifera
76000
-
recombinant enzyme, SDS-PAGE Apis mellifera

Organism

Organism UniProt Comment Textmining
Apis mellifera A8J4S9
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose Fast Flow column chromatography and Sephacryl S-100 gel filtration Apis mellifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
Apis mellifera D-glucose
-
?

Synonyms

Synonyms Comment Organism
acid trehalase
-
Apis mellifera
alpha,alpha trehalase
-
Apis mellifera
alpha,alpha trehalose glucohydrolase
-
Apis mellifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7
-
recombinant enzyme Apis mellifera

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
recombinant enzyme, stable under incubation at 40°C or lower for 15 min Apis mellifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
662
-
trehalose recombinant enzyme, in 50 mM sodium phosphate buffer, pH 6.6, with 0.01% Triton X-100, at 35°C Apis mellifera

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 recombinant enzyme Apis mellifera