Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.28 extracted from

  • Gibson, R.P.; Gloster, T.M.; Roberts, S.; Warren, R.A.; Storch de Gracia, I.; Garcia, A.; Chiara, J.L.; Davies, G.J.
    Molecular basis for trehalase inhibition revealed by the structure of trehalase in complex with potent inhibitors (2007), Angew. Chem., 46, 4115-4119.
    View publication on PubMed
Search for more literature for 3.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1-thiatrehazolin
-
 Escherichia coli
validoxylamine A
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 trehalose at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P13482
-
-

Purification (Commentary)

Purification (Comment) Organism
with PEG 3350 (25% w/v) and bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane (Bis-Tris)/HCl buffer (0.1 m, pH 6.5) Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
 Escherichia coli D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
GH37 trehalase
-
 Escherichia coli
Tre37A
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
199
-
 trehalose at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000009
-
 1-thiatrehazolin at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli
0.00001
-
 validoxylamine A at 37°C in sodium maleate buffer (75 mM, pH 5.5) Escherichia coli