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Literature summary for 3.2.1.28 extracted from

  • Wisser, G.; Guttenberger, M.; Hampp, R.; Nehls, U.
    Identification and characterization of an extracellular acid trehalase from the ectomycorrhizal fungus Amanita muscaria (2000), New Phytol., 146, 169-175.
    View publication on PubMed
Search for more literature for 3.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
sucrose competitive Amanita muscaria
validamycin competitive Amanita muscaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 alpha,alpha-trehalose
-
 Amanita muscaria

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Amanita muscaria 5618
-
extracellular
-
 Amanita muscaria
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 x * 135000, SDS-PAGE Amanita muscaria
165000
-
 gel filtration Amanita muscaria

Organism

Organism UniProt Comment Textmining
Amanita muscaria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Amanita muscaria

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Amanita muscaria
-
mycelium
-
 Amanita muscaria
-
mycorrhiza activity is strongly decreased in ectomycorrhizas Amanita muscaria
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O highly specific for Amanita muscaria alpha-D-glucopyranose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 135000, SDS-PAGE Amanita muscaria

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000045
-
 validamycin
-
 Amanita muscaria
15
-
 sucrose
-
 Amanita muscaria

pI Value

Organism Comment pI Value Maximum pI Value
Amanita muscaria
-
-
 4.2