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Literature summary for 3.2.1.28 extracted from
- Purification and characterization of acid trehalase from muscle of Ascaris suum (Nematoda) (2003), Comp. Biochem. Physiol. B, 136, 61-69.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, 28% inhibition | Ascaris suum |  |
| FeCl3 | 10 mM, 35% inhibition | Ascaris suum | |
| ZnSO4 | 10 mM, 34% inhibition | Ascaris suum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.6 | - |
alpha,alpha-trehalose | - |
Ascaris suum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 10 mM, activates 1.2fold | Ascaris suum | |
| iodoacetate | 10 mM, activates 1.3fold | Ascaris suum | |
| KNO3 | 10 mM, activates 2.2fold | Ascaris suum | |
| MgCl2 | 10 mM, activates 2.4fold | Ascaris suum | |
| NaCl | 10 mM, activates 1.4fold | Ascaris suum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Ascaris suum |
| 76000 | - |
nondenaturing-PAGE | Ascaris suum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ascaris suum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ascaris suum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Ascaris suum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.531 | - |
- |
Ascaris suum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha,alpha-trehalose + H2O | - |
Ascaris suum | alpha-D-glucopyranose + D-glucose | - |
? | |
| isomaltose + H2O | - |
Ascaris suum | alpha-D-glucopyranose | - |
? | |
| maltose + H2O | - |
Ascaris suum | alpha-D-glucopyranose + D-glucose | - |
? | |
| melizitose + H2O | weak | Ascaris suum | beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
| sucrose + H2O | - |
Ascaris suum | beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Ascaris suum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Ascaris suum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | 30°C: about 65% of maximal activity, 65°C: about 40% of maximal activity | Ascaris suum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min, stable | Ascaris suum |
| 60 | - |
15 min, 50% loss of activity | Ascaris suum |
| 65 | - |
15 min, almost complete inactivation | Ascaris suum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.9 | - |
- |
Ascaris suum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | 7.9 | pH 3.2: about 45% of maximal activity, pH 6.9: about 40% of maximal activity | Ascaris suum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ascaris suum | - |
- |
4.2 |
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