Protein Variants | Comment | Organism |
---|---|---|
D108K | mutant enzyme fails to bind Ca2+ or to undergo enzyme activation | Schizosaccharomyces pombe |
D97L | mutant enzyme fails to bind Ca2+ or to undergo enzyme activation | Schizosaccharomyces pombe |
additional information | Ntp1p elutes mainly in an inactive conformation instead of the dimeric or trimeric active form of the enzyme. Activation of the enzyme under different conditions depends upon binding through the Ca2+-binding motif as a prerequiste for correct enzyme oligomerization to its active form | Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | D97 and D108 in the conserved putative Ca2+-binding motif of Ntp1p appear to be responsible for this interaction. Strains expressing Ntp1p variants that are unable to bind Ca2+ partially resemble those devoid of the ntp1+ gene in terms of trehalose hyperaccumulation | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | Schizosaccharomyces pombe | neutral trehalase mobilizes trehalose accumulated by fungal cells as a protective and storage carbohydrate | alpha-D-glucopyranose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | - |
Schizosaccharomyces pombe | alpha-D-glucopyranose + D-glucose | - |
? | |
alpha,alpha-trehalose + H2O | neutral trehalase mobilizes trehalose accumulated by fungal cells as a protective and storage carbohydrate | Schizosaccharomyces pombe | alpha-D-glucopyranose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ntp1p | - |
Schizosaccharomyces pombe |