Literature summary for 3.2.1.28 extracted from

Nakano, H.; Moriwaki, M.; Washino, T.; Kino, T.; Yoshizumi, H.; Kitahara, S.
Purification and some properties of a trehalase from a green alga, Lobosphaera sp. (1994), Biosci. Biotechnol. Biochem., 58, 1430-1434.

Search for more literature for 3.2.1.28

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	weak inhibition	Lobosphaera sp.
Fe3+	-	Lobosphaera sp.
Mn2+	weak	Lobosphaera sp.
sucrose	25 mM, 33% inhibition; competitive	Lobosphaera sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	-	trehalose	-	Lobosphaera sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Lobosphaera sp.	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
400000	-	gel filtration	Lobosphaera sp.

Organism

Organism	UniProt	Comment	Textmining
Lobosphaera sp.	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Lobosphaera sp.

Purification (Commentary)

Purification (Comment)	Organism
-	Lobosphaera sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Lobosphaera sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trehalose + H2O	-	Lobosphaera sp.	D-glucose	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 180000-220000, SDS-PAGE	Lobosphaera sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Lobosphaera sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	10 min, pH 5.5, stable below	Lobosphaera sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Lobosphaera sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	9	5°C, 15 h, stable	Lobosphaera sp.

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)