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Literature summary for 3.2.1.28 extracted from
- Properties of a free and a solubilized form of bound alpha,alpha-trehalase purified from honey bee thorax (1975), Can. J. Biochem., 53, 1106-1117.
General Stability
| General Stability | Organism |
|---|---|
| 8 M urea, 30°C, 10 min, 25% loss of activity | Apis mellifera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 100 mM required for inhibition of more than 50% | Apis mellifera |  |
| p-aminophenyl-beta-D-glucoside | - |
Apis mellifera | |
| p-nitrophenyl-beta-D-glucoside | - |
Apis mellifera | |
| PCMB | 1 mM is required for inhibition of more than 50% | Apis mellifera | |
| phenyl-beta-D-glucoside | - |
Apis mellifera | |
| sucrose | - |
Apis mellifera | |
| Tris | - |
Apis mellifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.89 | - |
trehalose | - |
Apis mellifera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, SDS-PAGE | Apis mellifera |
| 65000 | - |
gel filtration | Apis mellifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apis mellifera | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | enzyme contains some type of bound lipid | Apis mellifera |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Apis mellifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thorax | free and bound enzyme form | Apis mellifera | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50 | - |
free enzyme | Apis mellifera |
| 53.4 | - |
solubilized enzyme | Apis mellifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | - |
Apis mellifera | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Apis mellifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
a minor activity maximum at pH 3.5 and a major activity maximum at pH 6.5 | Apis mellifera |
| 6.5 | - |
a minor activity maximum at pH 3.5 and a major activity maximum at pH 6.5 | Apis mellifera |
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Release 2023.1 (January 2023)
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