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Literature summary for 3.2.1.28 extracted from
- A comparison of thermal characteristics and kinetic parameters of trehalase from a thermophilic and a mesophilic fungus (1999), FEMS Microbiol. Lett., 181, 187-193.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
trehalose | - |
Neurospora crassa |  |
| 0.57 | - |
trehalose | - |
Thermomyces lanuginosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
4 * 92000, SDS-PAGE | Neurospora crassa |
| 145000 | - |
gel filtration | Thermomyces lanuginosus |
| 437000 | - |
gel filtration | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
| Thermomyces lanuginosus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 20% carbohydrate content | Thermomyces lanuginosus |
| glycoprotein | contains 43% carbohydrate | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
- |
Thermomyces lanuginosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 124 | - |
- |
Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | - |
Neurospora crassa | D-glucose | - |
? | |
| trehalose + H2O | - |
Thermomyces lanuginosus | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Thermomyces lanuginosus |
| tetramer | 4 * 92000, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Neurospora crassa |
| 50 | - |
- |
Thermomyces lanuginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable for at least 6 h | Neurospora crassa |
| 50 | - |
stable for at least 6 h | Thermomyces lanuginosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Thermomyces lanuginosus |
| 5 | 5.5 | - |
Neurospora crassa |
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