General Stability | Organism |
---|---|
enzyme form A1 becomes labile on addition of glycerol, activity in presence of 20% or 40% glycerol is about 30-40% lower than in absence of glycerol. Enzyme form B2 becomes more stable on addition of glycerol, activity in presence of 20% or 40% glycerol is about 30 higher than in absence of glycerol | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | the glycoprotein enzyme forms are more susceptible than the nonglycoprotein forms | Oryctolagus cuniculus | |
methyl beta-glucoside | - |
Oryctolagus cuniculus | |
phlorizin | - |
Oryctolagus cuniculus | |
Tris | - |
Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | the glycoprotein enzyme forms show lower activation than the nonglycoprotein forms | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
175000 | - |
gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
2 nonglycoprotein enzyme forms: A1 and B1 and 2 glycoprotein enzyme form A2 and B2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2 nonglycoprotein enzyme forms: A1 and B1, and 2 glycoprotein enzyme forms: A2 and B2 | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
4 enzyme form | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | cortex | Oryctolagus cuniculus | - |
Storage Stability | Organism |
---|---|
-80°C, 9 days, 15% loss of activity of enzyme form A1, 5% loss of activity of enzyme form A2, 35% loss of activity of enzyme form B1, 65% loss of activity of enzyme form B2 | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trehalose + H2O | - |
Oryctolagus cuniculus | D-glucose | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6 | phosphate buffer | Oryctolagus cuniculus |