Inhibitors | Comment | Organism | Structure |
---|---|---|---|
swainsonine | swainsonine treatment completely inhibits the acid enzyme form activity below pH 5.0 | Equus caballus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
4-methylumbelliferyl alpha-D-mannopyranoside | at pH 5.0 and 37°C | Equus caballus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Equus caballus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the neutral enzyme form is activated by Co2+ | Equus caballus | |
additional information | the acid enzyme form is not activated by Co2+ | Equus caballus | |
Zn2+ | the acid enzyme form is stabilized or stimulated by Zn2+ | Equus caballus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
154000 | - |
gel filtration | Equus caballus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Equus caballus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epididymal fluid | the activity of acid alpha-mannosidase I is highest in the epididymal fluid | Equus caballus | - |
spermatozoon | the activity of neutral alpha-mannosidase II is highest in mature spermatozoa | Equus caballus | - |
Storage Stability | Organism |
---|---|
-20°C, 7, 10 and 30 days, 20%, 30% and 40% loss of activity, respectively | Equus caballus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-mannopyranoside + H2O | - |
Equus caballus | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4 mannosidase | - |
Equus caballus |
alpha-D-mannosidase | - |
Equus caballus |
alpha-mannosidase I | acid enzyme form | Equus caballus |
alpha-mannosidase II | neutral enzyme form | Equus caballus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
80% of initial activity remains after 40-min incubation time at 60°C. After 60 min at this temperature, activity is lost | Equus caballus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4 | isoform alpha-mannosidase I | Equus caballus |
General Information | Comment | Organism |
---|---|---|
physiological function | the acid enzyme form alpha-mannosidase I plays a role in plasma membrane remodeling associated with sperm maturation. The neutral enzyme form alpha-mannosidase II acts as a receptor in the recognition and binding of the complementary carbohydrate moieties present on the zona pellucida | Equus caballus |