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Literature summary for 3.2.1.24 extracted from

  • Nielsen, J.W.; Poulsen, N.R.; Johnsson, A.; Winther, J.R.; Stipp, S.L.; Willemoes, M.
    Metal-ion dependent catalytic properties of Sulfolobus solfataricus class ii alpha-mannosidase (2012), Biochemistry, 51, 8039-8046.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibits in presence of Co2+ Saccharolobus solfataricus
Ni2+ inhibits in presence of Co2+ Saccharolobus solfataricus
swainsonine the enzyme substituted with Zn2+ is considerably less sensitive to swainsonine compared with the Co2+-, Cd2+-, and Mn2+-substituted enzyme Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
0.39
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
0.65
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
0.85
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
0.99
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
1
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay Saccharolobus solfataricus
1.16
-
alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
2
-
alpha-D-Man-(1->6)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
2
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
2.3
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
2.5
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus
Co2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus
Mn2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus
Zn2+ with alpha-1,2-, alpha-1,3-, alpha-1,4-, or alpha-1,6-mannobiose as a substrate, Co2+ is the only metal ion promoting hydrolysis of all substrates. Mn2+, Cd2+, and Zn2+ can substitute to a varying extent Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UK5
-
-
Saccharolobus solfataricus P2 Q97UK5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-mannoside + H2O
-
Saccharolobus solfataricus 4-nitrophenol + alpha-D-mannose
-
?
4-nitrophenyl alpha-D-mannoside + H2O
-
Saccharolobus solfataricus P2 4-nitrophenol + alpha-D-mannose
-
?
alpha-D-Man-(1->2)-D-Man + H2O
-
Saccharolobus solfataricus 2 alpha-D-mannose
-
?
alpha-D-Man-(1->2)-D-Man + H2O
-
Saccharolobus solfataricus P2 2 alpha-D-mannose
-
?
alpha-D-Man-(1->3)-D-Man + H2O
-
Saccharolobus solfataricus 2 alpha-D-mannose
-
?
alpha-D-Man-(1->3)-D-Man + H2O
-
Saccharolobus solfataricus P2 2 alpha-D-mannose
-
?
alpha-D-Man-(1->4)-D-Man + H2O
-
Saccharolobus solfataricus 2 alpha-D-mannose
-
?
alpha-D-Man-(1->4)-D-Man + H2O
-
Saccharolobus solfataricus P2 2 alpha-D-mannose
-
?
alpha-D-Man-(1->6)-D-Man + H2O
-
Saccharolobus solfataricus 2 alpha-D-mannose
-
?
alpha-D-Man-(1->6)-D-Man + H2O
-
Saccharolobus solfataricus P2 2 alpha-D-mannose
-
?

Synonyms

Synonyms Comment Organism
GH38 class II alpha-mannosidase
-
Saccharolobus solfataricus
ManA
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
2
-
alpha-D-Man-(1->6)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
3.1
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
4.8
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
4.9
-
alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
5.5
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
10.4
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
10.5
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay Saccharolobus solfataricus
12.5
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
13.6
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
16.1
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.102
-
alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
0.106
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
0.185
-
alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
0.206
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
0.277
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
0.28
-
alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
0.31
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
0.95
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
1
-
alpha-D-Man-(1->6)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
1.3
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
4.2
-
alpha-D-Man-(1->4)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
4.2
-
alpha-D-Man-(1->3)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
6.4
-
alpha-D-Man-(1->2)-D-Man pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus
10.5
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, untreated enzyme which with no externally added metal ion in the incubation assay Saccharolobus solfataricus
12
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Mn2+ Saccharolobus solfataricus
16
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Cd2+ Saccharolobus solfataricus
21
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Zn2+ Saccharolobus solfataricus
120
-
4-nitrophenyl alpha-D-mannoside pH 5.0, 70°C, enzyme in complex with Co2+ Saccharolobus solfataricus