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Literature summary for 3.2.1.24 extracted from

  • Ghosh, S.; Meli, V.S.; Kumar, A.; Thakur, A.; Chakraborty, N.; Chakraborty, S.; Datta, A.
    The N-glycan processing enzymes alpha-mannosidase and beta-D-N-acetylhexosaminidase are involved in ripening-associated softening in the non-climacteric fruits of capsicum (2011), J. Exp. Bot., 62, 571-582.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Capsicum annuum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
4-nitrophenyl-alpha-D-mannoside pH 6.0, 37°C Capsicum annuum

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Capsicum annuum 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
SDS-PAGE Capsicum annuum
290000
-
gel filtration, discrepancy in molecular mass due to glycoproteic nature Capsicum annuum
500000
-
non-denaturating PAGE Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Capsicum annuum

Purification (Commentary)

Purification (Comment) Organism
purification procedure consists of ammonium sulfate precipitation, anion exchange, ConA lectin affinity and gel filtration chromatography Capsicum annuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-mannoside + H2O
-
Capsicum annuum 4-nitrophenol + alpha-D-mannose
-
?

Subunits

Subunits Comment Organism
oligomer
-
Capsicum annuum

Synonyms

Synonyms Comment Organism
alpha-man
-
Capsicum annuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Capsicum annuum
55
-
optimal activity Capsicum annuum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable for 1 h Capsicum annuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Capsicum annuum

Expression

Organism Comment Expression
Capsicum annuum alpha-Man transcripts as well as enzyme activity increase with the ripening and/or softening of capsicum up

General Information

General Information Comment Organism
malfunction alpha-Man RNAi fruits are approximately two times firmer compared with the control and fruit deterioration is delayed by approximately 7 d. It is shown that silencing of alpha-Man enhances fruit shelf life due to the reduced degradation of N-glycoproteins which result in delayed softening Capsicum annuum