Cloned (Comment) | Organism |
---|---|
- |
Capsicum annuum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
4-nitrophenyl-alpha-D-mannoside | pH 6.0, 37°C | Capsicum annuum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Capsicum annuum | 5618 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
SDS-PAGE | Capsicum annuum |
290000 | - |
gel filtration, discrepancy in molecular mass due to glycoproteic nature | Capsicum annuum |
500000 | - |
non-denaturating PAGE | Capsicum annuum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capsicum annuum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Capsicum annuum |
Purification (Comment) | Organism |
---|---|
purification procedure consists of ammonium sulfate precipitation, anion exchange, ConA lectin affinity and gel filtration chromatography | Capsicum annuum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-mannoside + H2O | - |
Capsicum annuum | 4-nitrophenol + alpha-D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Capsicum annuum |
Synonyms | Comment | Organism |
---|---|---|
alpha-man | - |
Capsicum annuum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Capsicum annuum |
55 | - |
optimal activity | Capsicum annuum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 1 h | Capsicum annuum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Capsicum annuum |
Organism | Comment | Expression |
---|---|---|
Capsicum annuum | alpha-Man transcripts as well as enzyme activity increase with the ripening and/or softening of capsicum | up |
General Information | Comment | Organism |
---|---|---|
malfunction | alpha-Man RNAi fruits are approximately two times firmer compared with the control and fruit deterioration is delayed by approximately 7 d. It is shown that silencing of alpha-Man enhances fruit shelf life due to the reduced degradation of N-glycoproteins which result in delayed softening | Capsicum annuum |