Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Streptococcus pneumoniae |
expressed in Escherichia coli as a His-tagged fusion protein | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structures obtained in apo-, inhibitor-bound, and substrate-bound forms provide both mechanistic and molecular insight into how the proteins, which adopts an (alpha/alpha)6fold, recognizes and hydrolyzes the alpha1,6-mannosidic bond by an inverting, metal-independent catalytic mechanism | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Streptococcus pneumoniae |
using Ni-NTA chromatography | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O | - |
Streptococcus pneumoniae | 2,4-dinitrophenol + alpha-D-mannopyranose | - |
? | |
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O | - |
Clostridium perfringens | 2,4-dinitrophenol + alpha-D-mannopyranose | - |
? | |
additional information | enzyme processes alpha1,6-mannobiose into the product mannose. No activity on alpha1,2-mannobiose, alpha1,3-mannobiose or alpha(1,3)/(1,6)-mannotriose | Streptococcus pneumoniae | ? | - |
? | |
additional information | enzyme processes alpha1,6-mannobiose into the product mannose. No activity on alpha1,2-mannobiose, alpha1,3-mannobiose or alpha(1,3)/(1,6)-mannotriose | Clostridium perfringens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CpGH125 | - |
Clostridium perfringens |
SpGH125 | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay with 2,4-dinitrophenyl alpha-D-mannopyranoside | Streptococcus pneumoniae |
25 | - |
assay with 2,4-dinitrophenyl alpha-D-mannopyranoside | Clostridium perfringens |
37 | - |
assay at | Streptococcus pneumoniae |
37 | - |
assay at | Clostridium perfringens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Streptococcus pneumoniae |
7.4 | - |
assay at | Clostridium perfringens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00216 | - |
2,4-dinitrophenyl alpha-D-mannopyranoside | pH 7.4, 25°C | Streptococcus pneumoniae | |
0.00966 | - |
2,4-dinitrophenyl alpha-D-mannopyranoside | pH 7.4, 25°C | Clostridium perfringens |