Cloned (Comment) | Organism |
---|---|
expressed as a fusion of the glutathione S-transferase of Schistosoma japonicum in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D338G | inactive mutant enzyme. In presence of the external nucleophil (1 M) sodium formate the activity is partially rescued | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is insensitive to 1-deoxymannojirimycin at concentration up to 2 mM. No change in the enzymatic activity is observed with 0.001-1 mM EDTA | Saccharolobus solfataricus | |
swainsonine | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
4-nitrophenyl alpha-D-mannoside | pH 6.5, 65°C, recombinant enzyme | Saccharolobus solfataricus | |
0.45 | - |
4-nitrophenyl alpha-D-mannoside | pH 6.5, 65°C, native enzyme | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 0.01 and 0.1 mM, activates recombinant enzyme | Saccharolobus solfataricus | |
Co2+ | 0.01 and 0.1 mM, activates recombinant enzyme | Saccharolobus solfataricus | |
Mg2+ | 0.1 mM, activates recombinant enzyme | Saccharolobus solfataricus | |
Mn2+ | 1 mM, activates recombinant enzyme | Saccharolobus solfataricus | |
additional information | no change in the enzymatic activity is observed with Cu2+ or Ni2+ at 0.001-1 mM | Saccharolobus solfataricus | |
Zn2+ | the enzyme binds Zn2+ in vivo, it contains 2.9 Zn2+ per trimeric enzyme, corresponding to a single atom per subunit. 0.1 mM Zn2+ showes 2fold activation of recombinant enzyme | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
3 * 110000, SDS-PAGE | Saccharolobus solfataricus |
363000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharolobus solfataricus | the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein | ? | - |
? | |
additional information | Saccharolobus solfataricus P2 | the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97UK5 | - |
- |
Saccharolobus solfataricus P2 | Q97UK5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.4 | - |
substrate: 4-nitrophenyl alpha-D-mannoside, pH 6.5, 65°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-mannoside + H2O | no hydrolysis observed on 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-D-xyloside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl alpha-L-rhamnoside, 4-nitrophenyl alpha-L-fucoside, and 4-nitrophenyl beta-L-fucoside | Saccharolobus solfataricus | 4-nitrophenol + alpha-D-mannose | - |
? | |
4-nitrophenyl alpha-D-mannoside + H2O | no hydrolysis observed on 4-nitrophenyl alpha-D-glucoside, 4-nitrophenyl alpha-D-galactoside, 4-nitrophenyl alpha-D-xyloside, 4-nitrophenyl alpha-L-arabinoside, 4-nitrophenyl alpha-L-rhamnoside, 4-nitrophenyl alpha-L-fucoside, and 4-nitrophenyl beta-L-fucoside | Saccharolobus solfataricus P2 | 4-nitrophenol + alpha-D-mannose | - |
? | |
alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O | - |
Saccharolobus solfataricus | alpha-D-mannose + ? | - |
? | |
alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O | - |
Saccharolobus solfataricus P2 | alpha-D-mannose + ? | - |
? | |
alpha-D-Man-(1->2)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->2)-D-Man + H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->3)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->3)-D-Man + H2O | - |
Saccharolobus solfataricus P2 | 2 alpha-D-mannose | - |
? | |
alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O | - |
Saccharolobus solfataricus | alpha-D-mannose + ? | - |
? | |
alpha-D-Man-(1->6)-D-Man + H2O | - |
Saccharolobus solfataricus | 2 alpha-D-mannose | - |
? | |
additional information | the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein | Saccharolobus solfataricus P2 | ? | - |
? | |
RNase-B-Man(n)-glycoform + H2O | - |
Saccharolobus solfataricus | RNase-B-Man(n-1)-glycoform + alpha-D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 110000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
GH38 alpha-mannosidase | - |
Saccharolobus solfataricus |
Ssa-man | - |
Saccharolobus solfataricus |
SSO3006 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Saccharolobus solfataricus |
85 | - |
- |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 1.5 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
4-nitrophenyl alpha-D-mannoside | pH 6.5, 65°C, recombinant enzyme | Saccharolobus solfataricus | |
158 | - |
4-nitrophenyl alpha-D-mannoside | pH 6.5, 65°C, native enzyme | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.2 | - |
pH 6.5, 65°C | Saccharolobus solfataricus | swainsonine |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme could be putatively involved in the turnover and/or the maturation of glycoprotein | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54 | - |
4-nitrophenyl alpha-D-mannoside | pH 6.5, 65°C, recombinant enzyme | Saccharolobus solfataricus | |
351 | - |
4-nitrophenyl alpha-D-mannoside | pH 6.5, 65°C, native enzyme | Saccharolobus solfataricus |