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Literature summary for 3.2.1.24 extracted from

  • Shashidhara, K.S.; Gaikwad, S.M.
    Class II alpha-mannosidase from Aspergillus fischeri: energetics of catalysis and inhibition (2009), Int. J. Biol. Macromol., 44, 112-115.
    View publication on PubMed
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C Aspergillus fischeri
swainsonine binding of swainsonine to the enzyme is stronger than bionding of 1-deoxymannojirimycin; competitive inhibitor, low binding up to 40°C, increased binding at 50°C Aspergillus fischeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
 4-methylumbelliferyl-alpha-D-mannopyranoside pH 6.5, 50°C Aspergillus fischeri
0.00731
-
 4-methylumbelliferyl alpha-D-mannopyranoside pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3 Aspergillus fischeri
0.0826
-
 4-nitrophenyl-alpha-D-mannopyranoside pH 6.5, 50°C Aspergillus fischeri
0.08265
-
 4-nitrophenyl-alpha-D-mannopyranoside
-
 Aspergillus fischeri
0.839
-
 Manalpha(1-3)Man pH 6.5, 50°C Aspergillus fischeri
0.839
-
 Man-alpha(1-3)Man pH 6.5, 50°C, 20 min Aspergillus fischeri
1.116
-
 Manalpha(1-2)Man pH 6.5, 50°C Aspergillus fischeri
1.455
-
 Man-alpha(1-6)Man pH 6.5, 50°C Aspergillus fischeri
1.455
-
 Man-alpha(1-6)Man pH 6.5, 50°C, 20 min Aspergillus fischeri

Organism

Organism UniProt Comment Textmining
Aspergillus fischeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fischeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl alpha-D-mannopyranoside + H2O pH 6.5, 50°C, 15 min, reaction stopped with Na2CO3 Aspergillus fischeri 4-methylumbelliferone + alpha-D-mannose
-
 ?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
-
 Aspergillus fischeri 4-methylumbelliferone + alpha-D-mannopyranose
-
 ?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Aspergillus fischeri 4-nitrophenol + D-mannose
-
 ?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Aspergillus fischeri 4-nitrophenyl + alpha-D-mannose
-
 ?
Man-alpha(1-3)Man + H2O pH 6.5, 50°C, 20 min Aspergillus fischeri alpha-D-mannose
-
 ?
Man-alpha(1-6)Man + H2O pH 6.5, 50°C, 20 min Aspergillus fischeri alpha-D-mannose
-
 ?
Manalpha(1-2)Man + H2O
-
 Aspergillus fischeri D-mannose
-
 ?
Manalpha(1-3)Man + H2O
-
 Aspergillus fischeri D-mannose
-
 ?
Manalpha(1-6)Man + H2O
-
 Aspergillus fischeri D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
class II alpha-mannosidase
-
 Aspergillus fischeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus fischeri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 15 min Aspergillus fischeri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 fully stable, no inactivation, no denaturation Aspergillus fischeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Aspergillus fischeri

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.101
-
 swainsonine low binding up to 40°C, increased binding at 50°C Aspergillus fischeri
0.101
-
 swainsonine pH 6.5, 50°C Aspergillus fischeri
0.286
-
 1-deoxymannojirimycin low binding up to 40°C, increased binding at 50°C Aspergillus fischeri
0.286
-
 1-deoxymannojirimycin pH 6.5, 50°C Aspergillus fischeri

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.11
-
 low binding up to 40°C, increased binding at 50°C Aspergillus fischeri swainsonine
0.284
-
 low binding up to 40°C, increased binding at 50°C Aspergillus fischeri 1-deoxymannojirimycin