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Literature summary for 3.2.1.24 extracted from

  • Yamagishi, M.; Ishimizu, T.; Natsuka, S.; Hase, S.
    Co(II)-regulated substrate specificity of cytosolic alpha-mannosidase (2002), J. Biochem., 132, 253-256.
    View publication on PubMed
Search for more literature for 3.2.1.24

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 Man9GlcNAc pH 7.0, 37°C, Co2+-treated enzyme Gallus gallus
0.46
-
 Man9GlcNAc pH 7.0, 37°C, not treated with Co(II) Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Gallus gallus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, purified enzyme carries two Co2+ ions, the enzyme treated with Co2+ carries four. Co2+ regulates the substrate specificity of the enzyme. The Co2+-treated enzyme produces Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc and alpha-D-mannose as end products from Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc in presence of Co2+. Before tretament with Co2+ the enzyme is able to cleave a single Man-alpha-(1-2) residue from Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc to give Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha(1-3)]-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha(1-3)]-Man-beta-(1-4)-GlcNAc as the end product Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O Gallus gallus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
-
 Gallus gallus ?
-
 ?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
-
 Gallus gallus Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha(1-3)]-Man-beta-(1-4)-GlcNAc + alpha-mannose end product in presence of Co2+. Before treatment with Co(II) the enzyme is able to cleave a single Manalpha1-2 residue from the substrate to give Man-alpha(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]Man-beta-(1-4)-GlcNAc as the end product ?
p-nitrophenyl alpha-mannoside + H2O
-
 Gallus gallus p-nitrophenol + alpha-mannose
-
 ?