General Stability | Organism |
---|---|
glutathione S-transferase/mannosidase III fusion protein is inactivated by freezing | Spodoptera frugiperda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, more than 90% inactivation | Spodoptera frugiperda | |
swainsonine | IC50: 10 nM | Spodoptera frugiperda | |
ZnCl2 | 92% inhibition by 1 mM, 56% inhibition by 0.01 mM | Spodoptera frugiperda |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Spodoptera frugiperda | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM enhances activity 30.2fold | Spodoptera frugiperda | |
Mn2+ | 1 mM enhances activity 6fold | Spodoptera frugiperda | |
Ni2+ | 0.1 mM, enhances activity to 119% of control | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione S-transferase/mannosidase III fusion protein | Spodoptera frugiperda |
Storage Stability | Organism |
---|---|
4°C, glutathione S-transferase/mannosidase III fusion protein is stable for at least 1 week | Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man5GlcNAc2 + 2 H2O | - |
Spodoptera frugiperda | Man3GlcNAc2 + 2 mannose | - |
? | |
Man9GlcNAc2 + H2O | - |
Spodoptera frugiperda | Man8GlcNAc2 + mannose | - |
? | |
additional information | no hydrolysis of GlcNAcMan5GlcNAc2 | Spodoptera frugiperda | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | SDS-PAGE reveals a major protein of 150000 Da and a minor protein with a MW of 130000 Da | Spodoptera frugiperda |
Synonyms | Comment | Organism |
---|---|---|
alpha-mannosidase III | - |
Spodoptera frugiperda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.7 | - |
Spodoptera frugiperda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.7 | pH 5.7: about 35% of maximal activity, pH 6.5-6.7: optimum | Spodoptera frugiperda |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00001 | - |
IC50: 10 nM | Spodoptera frugiperda | swainsonine |