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Literature summary for 3.2.1.24 extracted from

  • Yamamoto, K.; Hitomi, J.; Kobatake, K.; Yamaguchi, H.
    Purification and characterization of 1,2-alpha-mannosidase of Aspergillus oryzae (1982), J. Biochem., 91, 1971-1979.
    View publication on PubMed
Search for more literature for 3.2.1.24

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine slight stimulation at 1 or 10 mM Aspergillus oryzae
NaN3 70% stimulation at 10 mM Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 52% inhibition at 10 mM Aspergillus oryzae
Ba2+ 10 mM; 78% inhibition at 10 mM Aspergillus oryzae
Cd2+ 10 mM, activation at 1 mM; 54% inhibition at 10 mM, slight stimulation at 1 mM Aspergillus oryzae
Co2+ 51% inhibition at 10 mM Aspergillus oryzae
Cu2+ 10 mM; 41% inhibition at 10 mM Aspergillus oryzae
D-mannono-1,4-lactone 36% inhibition at 10 mM Aspergillus oryzae
D-mannono-gamma-lactone
-
 Aspergillus oryzae
EDTA slight inhibition at 1 mM Aspergillus oryzae
Fe2+ 58% inhibition at 10 mM Aspergillus oryzae
Fe3+ 43% inhibition at 1 mM Aspergillus oryzae
Hg2+ complete inhibition at 10 mM Aspergillus oryzae
iodoacetic acid
-
 Aspergillus oryzae
methyl-alpha-mannoside not Aspergillus oryzae
additional information no inhibition by L-cysteine Aspergillus oryzae
Na+ 1 mM, weak, not at 10 mM Aspergillus oryzae
Zn2+ 55% inhibition at 10 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 2-O-alpha-D-mannopyranosyl-D-mannopyranose
-
 Aspergillus oryzae
0.57
-
 2-O-alpha-D-Mannopyranosyl-D-mannose 37°C, pH 5.3 Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activation, 1 mM, inhibits at 10 mM Aspergillus oryzae
Mg2+ not Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O Aspergillus oryzae
-
 2 alpha-D-mannopyranose
-
 ?
glycopeptide + H2O Aspergillus oryzae from ovalbumin alpha-D-mannopyranose + ?
-
 ?
glycopeptide + H2O Aspergillus oryzae contains only alpha-1,2-linked mannose residues, highly specific on alpha-1,2-linked mannooligosaccharides alpha-D-mannopyranose + ?
-
 ?
mannan + H2O Aspergillus oryzae from yeast alpha-D-mannose + ? only mannose librated ?
ribonuclease B + H2O Aspergillus oryzae from bovine pancreas oligosaccharide + alpha-D-mannopyranose + ? single oligosaccharide component ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus oryzae
further purification of TAKA-diastase, to homogeneity Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation TAKA diastase, crude enzyme preparation Aspergillus oryzae
-
commercial preparation Taka-diastase, enzyme-product from Sankyo Co. Aspergillus oryzae
-

Storage Stability

Storage Stability Organism
4°C, pH 4.5-6, at least 6 months Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
-
 Aspergillus oryzae alpha-D-mannopyranose + D-mannopyranose
-
 ?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
-
 Aspergillus oryzae 2 alpha-D-mannopyranose
-
 ?
2-O-alpha-D-mannopyranosyl-D-mannose + H2O
-
 Aspergillus oryzae D-mannose
-
 ?
baker's yeast mannan + H2O releases 9.2% of total mannose Aspergillus oryzae ? + D-mannose
-
 ?
glycopeptide + H2O from ovalbumin Aspergillus oryzae alpha-D-mannopyranose + ?
-
 ?
glycopeptide + H2O contains only alpha-1,2-linked mannose residues, highly specific on alpha-1,2-linked mannooligosaccharides Aspergillus oryzae alpha-D-mannopyranose + ?
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O highly active on alpha(1-2) linked mannooligosaccharides Aspergillus oryzae (Man)6(GlcNAc)2 + D-mannose
-
 ?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. GPIV Aspergillus oryzae (Man)5(GlcNAc)2-Asn + D-mannose
-
 ?
mannan + H2O from yeast Aspergillus oryzae alpha-D-mannose + ? only mannose librated ?
methyl-2,4,6-tri-O-methyl-D-mannopyranoside + H2O
-
 Aspergillus oryzae 2,4,6-tri-O-methyl-D-mannopyranose + methanol
-
 ?
additional information no substrate: 3-O-alpha- and 6-O-alpha-D-mannobioses Aspergillus oryzae ?
-
 ?
ribonuclease B + H2O from bovine pancreas Aspergillus oryzae oligosaccharide + alpha-D-mannopyranose + ? single oligosaccharide component ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10% loss of activity after 1 h Aspergillus oryzae
50
-
 1 h, in 0.1 M acetate buffer, pH 5, 10% loss of activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9 5.3
-
 Aspergillus oryzae
4.9 5.3 2-O-alpha-D-mannopyranosyl-D-mannose as substrate Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
4 5.8 about half-maximal activity at pH 4 and 5.8, 0.1 M acetate buffer Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6 stable for 6 months at 4°C Aspergillus oryzae