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Literature summary for 3.2.1.24 extracted from
- Purification and characterization of alpha-mannosidase and beta-N-acetylhexosaminidase from watermelon fruit (1988), Agric. Biol. Chem., 52, 2223-2230.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| albumin protects activity | Citrullus lanatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | addition of Zn2+ restores inhibitory effect | Citrullus lanatus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Citrullus lanatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 270000 | - |
gel filtration | Citrullus lanatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | Citrullus lanatus | from ovalbumin | alpha-D-mannopyranose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrullus lanatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Citrullus lanatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Citrullus lanatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Citrullus lanatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycopeptide + H2O | from ovalbumin | Citrullus lanatus | alpha-D-mannopyranose + ? | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Citrullus lanatus | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Citrullus lanatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | stable within | Citrullus lanatus |
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