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Literature summary for 3.2.1.24 extracted from
- Solubilization and purification of alpha-mannosidase, a marker-enzyme of vacuolar membranes in Saccharomyces cerevisiae (1988), J. Biol. Chem., 263, 5158-5163.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | nearly complete inhibition at 0.01 mM | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Saccharomyces cerevisiae | |
| 0.14 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound to vacuolar tonoplast | Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 560000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Saccharomyces cerevisiae | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | composed of one to six 107, 73 and 31 kDa subunits | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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