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Literature summary for 3.2.1.24 extracted from
- The alpha-mannosidase from Canavalia ensiformes seeds: chemical and kinetic properties and action on animal lymphocytes (1988), Biol. Chem. Hoppe-Seyler, 369, 165-169.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-mannopyranose | competitive inhibition, Ki: 22 mM | Canavalia ensiformis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
p-nitrophenyl-alpha-D-mannopyranoside | fairly constant between pH 4.5-6.0 | Canavalia ensiformis | |
| 6.5 | - |
p-nitrophenyl-alpha-D-mannopyranoside | at pH 7.0 | Canavalia ensiformis | |
| 10.9 | - |
p-nitrophenyl-alpha-D-mannopyranoside | at pH 3.5 | Canavalia ensiformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
alpha2, 2 * 44000, beta2, 2 * 66000, SDS-PAGE | Canavalia ensiformis |
| 66000 | - |
alpha2, 2 * 44000, beta2, 2 * 66000, SDS-PAGE | Canavalia ensiformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canavalia ensiformis | - |
jack bean, stimulates proliferation of B-lymphocytes from mice | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 6% carbohydrate content for the 66 kDa subunit, smaller subunit not glycosylated | Canavalia ensiformis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Canavalia ensiformis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Canavalia ensiformis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Canavalia ensiformis | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2, 2 * 44000, beta2, 2 * 66000, SDS-PAGE | Canavalia ensiformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 130 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Canavalia ensiformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.2 | - |
Canavalia ensiformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | - |
Canavalia ensiformis |
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