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Literature summary for 3.2.1.24 extracted from
- Identification and characterization of an alpha-mannosidase from Trypanosoma cruzi (1992), Glycobiology, 2, 563-569.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| swainsonine | complete inhibition at 0.008 mM | Trypanosoma cruzi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | - |
Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
4 * 58000, SDS-PAGE | Trypanosoma cruzi |
| 240000 | - |
gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
lysosomal type | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | only N-linked high-mannose-type oligosaccharides | Trypanosoma cruzi |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.33 | - |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Trypanosoma cruzi | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Trypanosoma cruzi | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 58000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Trypanosoma cruzi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
40% remaining activity | Trypanosoma cruzi |
| 4.5 | - |
45% remaining activity | Trypanosoma cruzi |
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