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Literature summary for 3.2.1.24 extracted from

  • Grarg, T.; Saint-Pol, A.; Haeuw, J.F.; Alonso, C.; Wieruszeski, J.M.; Strecker, G.; Michalski, J.C.
    Soluble forms of alpha-D-mannosidases from rat liver. Separation and characterization of two enzymic forms with different substrate specificities (1994), Eur. J. Biochem., 223, 99-106.
    View publication on PubMed
Search for more literature for 3.2.1.24

Inhibitors

Inhibitors Comment Organism Structure
swainsonine inhibition of both Tris-eluted and unbound isoforms from cobalt-chelating Sepharose, unbound: Ki: 0.028 mM, Tris-eluted: Ki: 0.004 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
 p-nitrophenyl-alpha-D-mannopyranoside Tris-eluted isoform from cobalt-chelating Sepharose Rattus sp.
0.63
-
 p-nitrophenyl-alpha-D-mannopyranoside unbound isoform from cobalt-chelating Sepharose Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation for Tris-eluted isoform from cobalt-chelating Sepharose Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
113000
-
 Tris-eluted isoform from cobalt-chelating Sepharose, gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Man9GlcNAc + 3 H2O Rattus sp.
-
 Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose products from unbound fraction of cobalt-chelating Sepharose ?
Man9GlcNAc + 4 H2O Rattus sp.
-
 Man5GlcNAc + 4 alpha-D-mannopyranose end product for Tris-eluted isoform, representing the Man5-oligosaccharide chain of the dolichol pathway formed in the cytosolic compartment during biosynthesis of N-glycosylprotein glycans ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, cobalt-chelating Sepharose Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver 2 isoforms, unbound and Tris-eluted fractions from cobalt-chelating Sepharose Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.42
-
 for Tris-eluted isoform Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Man9GlcNAc + 3 H2O
-
 Rattus sp. Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose products from unbound fraction of cobalt-chelating Sepharose ?
Man9GlcNAc + 4 H2O
-
 Rattus sp. Man5GlcNAc + 4 alpha-D-mannopyranose end product for Tris-eluted isoform, representing the Man5-oligosaccharide chain of the dolichol pathway formed in the cytosolic compartment during biosynthesis of N-glycosylprotein glycans ?
p-nitrophenyl-alpha-D-mannopyranoside + H2O different affinities for two isoforms, unbound fraction and Tris-eluted fraction from cobalt-chelating Sepharose Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 113000, gel filtration, SDS-PAGE Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.8 both isoforms, unbound and Tris-eluted fractions from cobalt-chelating Sepharose Rattus sp.