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Literature summary for 3.2.1.24 extracted from

  • Yamashiro, K.; Itoh, H.; Yamagishi, M.; Natsuka, S.; Mega, T.; Hase, S.
    Purification and characterization of neutral alpha-mannosidase from hen oviduct: studies on the activation mechanism of Co2+ (1997), J. Biochem., 122, 1174-1181.
    View publication on PubMed

General Stability

General Stability Organism
stable in the presence of Co2+ at 37°C Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimicin 19% inhibition at 0.01 mM Gallus gallus
Cu2+ complete inhibition of the Co2+ activated enzyme at 0.5 mM Gallus gallus
Pb2+ complete inhibition of the Co2+ activated enzyme at 0.5 mM Gallus gallus
swainsonine complete inhibition of the Co2+ activated enzyme at 0.01 mM Gallus gallus
Zn2+ complete inhibition of the Co2+ activated enzyme at 0.5 mM Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc
-
Gallus gallus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 24fold activation after preincubation with 1 mM Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
4 * 110000, SDS-PAGE Gallus gallus
480000
-
gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man9GlcNAc + H2O Gallus gallus Co2+ activated alpha-mannosidase Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose end products ?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O Gallus gallus 4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc alpha-D-mannopyranose + ?
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O Gallus gallus
-
alpha-D-mannopyranose + ?
-
?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
chicken
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
-
Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man9GlcNAc + H2O Co2+ activated alpha-mannosidase Gallus gallus Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose end products ?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O 4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc Gallus gallus alpha-D-mannopyranose + ?
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Gallus gallus alpha-D-mannopyranose + ?
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Gallus gallus p-nitrophenol + alpha-D-mannopyranose
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 110000, SDS-PAGE Gallus gallus

Synonyms

Synonyms Comment Organism
neutral alpha-mannosidase
-
Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Gallus gallus