General Stability | Organism |
---|---|
stable in the presence of Co2+ at 37°C | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxymannojirimicin | 19% inhibition at 0.01 mM | Gallus gallus | |
Cu2+ | complete inhibition of the Co2+ activated enzyme at 0.5 mM | Gallus gallus | |
Pb2+ | complete inhibition of the Co2+ activated enzyme at 0.5 mM | Gallus gallus | |
swainsonine | complete inhibition of the Co2+ activated enzyme at 0.01 mM | Gallus gallus | |
Zn2+ | complete inhibition of the Co2+ activated enzyme at 0.5 mM | Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc | - |
Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 24fold activation after preincubation with 1 mM | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
4 * 110000, SDS-PAGE | Gallus gallus |
480000 | - |
gel filtration | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man9GlcNAc + H2O | Gallus gallus | Co2+ activated alpha-mannosidase | Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose | end products | ? | |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O | Gallus gallus | 4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc | alpha-D-mannopyranose + ? | - |
? | |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | Gallus gallus | - |
alpha-D-mannopyranose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
chicken | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oviduct | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11 | - |
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man9GlcNAc + H2O | Co2+ activated alpha-mannosidase | Gallus gallus | Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose | end products | ? | |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O | 4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc | Gallus gallus | alpha-D-mannopyranose + ? | - |
? | |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Gallus gallus | alpha-D-mannopyranose + ? | - |
? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Gallus gallus | p-nitrophenol + alpha-D-mannopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 110000, SDS-PAGE | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
neutral alpha-mannosidase | - |
Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Gallus gallus |