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Literature summary for 3.2.1.24 extracted from

  • Merkle, R.K.; Zhang, Y.; Ruest, P.J.; Lal, A.; Liao, Y.F.; Moremen, K.W.
    Cloning, expression, purification, and characterization of the murine lysosomal acid alpha-mannosidase (1997), Biochim. Biophys. Acta, 1336, 132-146.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
swainsonine IC50: 0.00015 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.6
-
p-nitrophenyl-alpha-D-mannopyranoside
-
Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
670000
-
gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-mannosidosis fibroblasts + H2O Mus musculus
-
Manbeta(1-4)GlcNAc + ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus O09159
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, to homogeneity Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
liver cDNA library Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.86
-
-
Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6 pyridylamino-Man9GlcNAc2 + H2O stepwise cleavage to Man2GlcNAc structures Mus musculus pyridylamino-Man8GlcNAc2 + pyridylamino-Man7GlcNAc2 + pyridylamino-Man6GlcNAc2 + pyridylamino-Man5GlcNAc2 + pyridylamino-Man4GlcNAc2 + pyridylamino-Man3GlcNAc2 + 21 alpha-D-mannopyranose
-
?
alpha-D-mannosidosis fibroblasts + H2O
-
Mus musculus Manbeta(1-4)GlcNAc + ?
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Mus musculus p-nitrophenol + alpha-D-mannopyranose
-
?
pyridylamino-Man5GlcNAc + H2O
-
Mus musculus pyridylamino-Man4GlcNAc + pyridylamino-Man3-2GlcNAc + alpha-D-mannose lesser degree of pyridylamino-Man3-2GlcNAc, stepwise cleavage to Man2GlcNAc structures ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6
-
Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00015
-
IC50: 0.00015 mM Mus musculus swainsonine