Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
swainsonine | 50% inhibition at 0.00024 mM | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Trypanosoma cruzi | |
5.2 | - |
4-methylumbelliferyl-alpha-D-mannopyranoside | - |
Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
359000 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | presence of N-glycans | Trypanosoma cruzi |
Purification (Comment) | Organism |
---|---|
recombinant protein, to homogeneity | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.72 | - |
- |
Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Trypanosoma cruzi | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
Manalpha(1-2)Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + H2O | - |
Trypanosoma cruzi | Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-4)anhydromannitol + anhydromannitol + alpha-D-mannose | equal amounts, but only traces of Manalpha(1-4)-anhydromannitol | ? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Trypanosoma cruzi | p-nitrophenol + alpha-D-mannopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | heterotetramer composed of 57000 + 46000 subunits, SDS-PAGE, endo H deglycosylation | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
acid alpha-mannosidase | - |
Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00024 | - |
swainsonine | - |
Trypanosoma cruzi |