Application | Comment | Organism |
---|---|---|
synthesis | the F441Y mutant enzyme has potential application in the industrial preparation of galactooligosaccharides | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
mutant F441Y is expressed in Pichia pastoris | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
F441Y | mutant enzyme shows good thermostability | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.3 | - |
lactose | pH 7.5, 80°C, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P22498 | - |
- |
Saccharolobus solfataricus P2 | P22498 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme F441Y | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
152.3 | - |
pH 7.5, 80°C, activity of recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus P2 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Saccharolobus solfataricus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Saccharolobus solfataricus P2 | D-glucose + D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
LACS | - |
Saccharolobus solfataricus |
SSO3019 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 95 | 65°C; about 55% of maximal activity, 95°C: about 90% of maximal activity, recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
50% loss of activity after 12 h, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
80 | - |
50% loss of activity after 11 h, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
85 | - |
50% loss of activity after 5.5 h, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.67 | - |
lactose | pH 7.5, 80°C, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | pH 4.5: about 85% of maximal activity, pH 7.5: about 50% of maximal activity, recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
half-life: 30 h | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
lactose | pH 7.5, 80°C, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |