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Literature summary for 3.2.1.23 extracted from
- Characterization of an extremely thermostable but cold-adaptive beta-galactosidase from the hyperthermophilic archaeon Pyrococcus furiosus for use as a recombinant aggregation for batch lactose degradation at high temperature (2014), J. Biosci. Bioeng., 117, 706-710.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the recombinant thermostable beta-galactosidase may be suitable for the hydrolysis of lactose in milk processing | Pyrococcus furiosus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21. The active inclusion bodies of beta-galactosidase are easily isolated by nonionic detergent treatment and directly used for lactose conversion in a repetitive batch mode | Pyrococcus furiosus |
General Stability
| General Stability | Organism |
|---|---|
| when the recombinant enzyme from active inclusion bodies is utilized for lactose conversion in a repetitive batch mode, more than 54% (90°C) or 88% (10°C) of the original enzyme activity is retained after 10 conversion cycles under optimum conditions | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5 mM, inhibits the enzyme activity by 22.7%, 80°C, pH 7.0 | Pyrococcus furiosus |  |
| Cu2+ | 5 mM, inhibits the enzyme activity by 99.71%, 80°C, pH 7.0 | Pyrococcus furiosus | |
| Fe3+ | 5 mM, inhibits the enzyme activity by 95.2%, 80°C, pH 7.0 | Pyrococcus furiosus | |
| Mn2+ | 5 mM, inhibits the enzyme activity by 24.8%, 80°C, pH 7.0 | Pyrococcus furiosus | |
| Ni2+ | 5 mM, inhibits the enzyme activity by 92.9%, 80°C, pH 7.0 | Pyrococcus furiosus | |
| Zn2+ | 5 mM, inhibits the enzyme activity by 97.3%, 80°C, pH 7.0 | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 5 mM, 1.1fold stimulation of activity, 80°C, pH 7.0 | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59450 | - |
x * 59450, SDS-PAGE | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | E7FHC0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.2 | - |
pH 7, 0°C | Pyrococcus furiosus |
| 130 | - |
pH 7, 90°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Pyrococcus furiosus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | 94% of the activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| lactose + H2O | - |
Pyrococcus furiosus | D-glucose + D-galactose | - |
? | |
| additional information | activity with 4-nitrophenyl alpha-D-glucopyranoside is 0.26% of the activity compared to 2-nitrophenyl beta-D-galactopyranoside, activity with 4-nitrophenyl alpha-D-glucopyranoside is 10.91% of the activity compared to 2-nitrophenyl beta-D-galactopyranoside | Pyrococcus furiosus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59450, SDS-PAGE | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lactase | - |
Pyrococcus furiosus |
| PF1208 | locus name | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | 60°C: about 50% of maximal activity, 100°C: about 95% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 7.0, half-life: 31423.4 min | Pyrococcus furiosus |
| 80 | - |
pH 7.0, half-life: 8168.3 min | Pyrococcus furiosus |
| 85 | - |
pH 7.0, half-life: 4017.7 min | Pyrococcus furiosus |
| 90 | - |
pH 7.0, half-life: 547.4 min | Pyrococcus furiosus |
| 95 | - |
pH 7.0, half-life: 309.6 min | Pyrococcus furiosus |
| 100 | - |
pH 7.0, half-life: 203.5 min | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in phosphate buffer | Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | pH 6.5: about 60% of maximal activity, pH 7.5: about 50% of maximal activity | Pyrococcus furiosus |
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