Crystallization (Comment) | Organism |
---|---|
mutant enzymes H418E and H418N | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H418E | the mutant shows low catalytic activity | Escherichia coli |
H418F | the mutant shows low catalytic activity, the variant has reduced stability, binds Mg2+ weakly and cannot be crystallized | Escherichia coli |
H418N | the mutant shows low catalytic activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-2-deoxy-D-galactopyranose | - |
Escherichia coli | |
2-nitrophenyl 1-thio-beta-D-galactopyranoside | - |
Escherichia coli | |
2-phenylethyl 1-thio-beta-D-galactopyranoside | - |
Escherichia coli | |
D-galactonolactone | - |
Escherichia coli | |
D-galactose | - |
Escherichia coli | |
isopropyl-thio-beta-D-galactopyranoside | - |
Escherichia coli | |
L-arabinose | - |
Escherichia coli | |
L-ribose | - |
Escherichia coli | |
lactose | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
0.04 | - |
2-nitrophenyl beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C | Escherichia coli | |
0.06 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
0.12 | - |
4-nitrophenyl beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C | Escherichia coli | |
13.2 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
20.8 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
30.6 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
104 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the active site of beta-galactosidase contains a Mg2+ ion ligated by Glu-416, His-418 and Glu-461 plus three water molecules | Escherichia coli | |
Na+ | the active site of beta-galactosidase contains a Na+ ion | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Escherichia coli | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00722 | - |
- |
Purification (Comment) | Organism |
---|---|
Superose 6 gel filtration and Superose 12 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 2-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 4-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
beta-gal | - |
Escherichia coli |
BGA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
5.4 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
5.4 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
5.5 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
10.3 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
10.4 | - |
2-nitrophenyl beta-D-galactopyranoside | mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C | Escherichia coli | |
90 | - |
2-nitrophenyl beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C | Escherichia coli | |
620 | - |
4-nitrophenyl beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
2-phenylethyl 1-thio-beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.025 | - |
2-nitrophenyl 1-thio-beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.08 | - |
2-phenylethyl 1-thio-beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.08 | - |
isopropyl-thio-beta-D-galactopyranoside | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.28 | - |
L-ribose | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.4 | - |
D-galactonolactone | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.4 | - |
D-galactonolactone | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.64 | - |
D-galactonolactone | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.68 | - |
L-ribose | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
0.9 | - |
lactose | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
1 | - |
2-amino-2-deoxy-D-galactopyranose | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
1.3 | - |
2-amino-2-deoxy-D-galactopyranose | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
1.4 | - |
L-ribose | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
1.4 | - |
2-amino-2-deoxy-D-galactopyranose | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
2 | 5 | L-arabinose | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
3.3 | - |
D-galactose | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
3.4 | - |
D-galactose | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
4.2 | - |
2-nitrophenyl 1-thio-beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
5 | - |
isopropyl-thio-beta-D-galactopyranoside | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
5.1 | - |
lactose | mutant enzyme H418N, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
8.4 | - |
2-nitrophenyl 1-thio-beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
10 | - |
2-phenylethyl 1-thio-beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
13 | - |
lactose | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
21 | - |
D-galactose | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
85 | - |
isopropyl-thio-beta-D-galactopyranoside | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
130 | - |
L-arabinose | mutant enzyme H418E, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli | |
220 | - |
L-arabinose | wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, 0.01 mM Mg2+, pH 7.0 at 25°C | Escherichia coli |