Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Escherichia coli | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
refolding buffer (pH 8.0) contains 10 mM MgCl2, 0.01% Tween 20, 2.5 mM dithiothreitol, and 100 mM Tris-HCl. In a binary system using only L-Arg-HCl the refolding yield of beta-gal increases with increasing concentration up to 0.2 M, in a ternary system using both 0.2 M L-Arg-HCl and another salt, the refolding yield increases up to 1.5fold higher than that in the binary system | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
beta-gal | - |
Escherichia coli |