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Literature summary for 3.2.1.23 extracted from

  • Huang, S.F.; Liu, D.B.; Zeng, J.M.; Yuan, Y.; Xiao, Q.; Sun, C.M.; Li, C.L.; Tao, K.; Wen, J.P.; Huang, Z.G.; Feng, W.L.
    Cloning, expression, purification, distribution and kinetics characterization of the bacterial beta-galactosidase fused to the cytoplasmic transduction peptide in vitro and in vivo (2009), Protein Expr. Purif., 68, 167-176.
    View publication on PubMed
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120800
-
 thioredoxin-His portion removed cytoplasmic transduction peptide-beta-gal recombinant protein, calculated from amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Homo sapiens
-
 D-galactose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
-
 Homo sapiens 5-bromo-4-chloroindol + beta-D-galactopyranose
-
 ?
lactose + H2O
-
 Homo sapiens D-galactose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
beta-gal
-
 Homo sapiens
CTP-beta-gal CTP-fused beta-galactosidase Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens thioredoxin-His portion removed cytoplasmic transduction peptide-beta-gal recombinant protein, calculated from amino acid sequence
-
 5.6