Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | the lower concentrations (1-10 mM) of 2-mercaptoethanol show a stimulating effect on activity | Lactiplantibacillus plantarum WCFS1 | |
dithiothreitol | the lower concentrations (1-10 mM) of dithiothreitol show a stimulating effect on activity | Lactiplantibacillus plantarum WCFS1 | |
Urea | the lower concentrations (1-10 mM) of urea show a stimulating effect on activity | Lactiplantibacillus plantarum WCFS1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 100 mM of beta-mercaptoethanol shows an inhibitory effect on activity (91% residual activity) | Lactiplantibacillus plantarum WCFS1 | |
dithiothreitol | 100 mM of dithiothreitol shows an inhibitory effect on activity (84% residual activity) | Lactiplantibacillus plantarum WCFS1 | |
EDTA | EDTA strongly inactivates the enzyme even at 1 mM (17% residual activity), 13% residual activity at 100 mM EDTA | Lactiplantibacillus plantarum WCFS1 | |
Urea | 100 mM of urea shows an inhibitory effect on activity (48% residual activity) | Lactiplantibacillus plantarum WCFS1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 7.5 and 55°C | Lactiplantibacillus plantarum WCFS1 | |
29 | - |
lactose | at pH 7.0 and 60°C | Lactiplantibacillus plantarum WCFS1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 326% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
Mg2+ | the enzyme needs MgCl2 for its stability retaining 73% and 85% of its activity after an incubation at 37°C for 5 h in the presence of 1 and 10 mM MgCl2, 414% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
Mn2+ | 341% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 | |
Na+ | 165% relative activity at 10 mM | Lactiplantibacillus plantarum WCFS1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
35223 | - |
1 * 72180 + 1 * 35223, calculated from amino acid sequence | Lactiplantibacillus plantarum WCFS1 |
72000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
72180 | - |
1 * 72180 + 1 * 35223, calculated from amino acid sequence | Lactiplantibacillus plantarum WCFS1 |
107000 | - |
gel filtration | Lactiplantibacillus plantarum WCFS1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum WCFS1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
p-aminobenzyl 1-thio-beta-D-galactopyranoside column chromatography | Lactiplantibacillus plantarum WCFS1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | preferred substrate | Lactiplantibacillus plantarum WCFS1 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | preferred substrate | Lactiplantibacillus plantarum WCFS1 | D-galactose + D-glucose | - |
? | |
additional information | the enzyme shows a strong preference for the formation of beta-(1-6) linkages in its transgalactosylation mode, while beta-(1-3)-linked products are formed to a lesser extent, comprising about 80% and 9%, respectively, of the newly formed glycosidic linkages in the oligosaccharide mixture at maximum galacto-oligosaccharides formation | Lactiplantibacillus plantarum WCFS1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 72000 + 1 * 35000, SDS-PAGE | Lactiplantibacillus plantarum WCFS1 |
heterodimer | 1 * 72180 + 1 * 35223, calculated from amino acid sequence | Lactiplantibacillus plantarum WCFS1 |
Synonyms | Comment | Organism |
---|---|---|
beta-D-galactoside galactohydrolase | - |
Lactiplantibacillus plantarum WCFS1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside | Lactiplantibacillus plantarum WCFS1 |
60 | - |
for hydrolysis of lactose | Lactiplantibacillus plantarum WCFS1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
92 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 7.5 and 55°C | Lactiplantibacillus plantarum WCFS1 | |
98 | - |
lactose | at pH 7.0 and 60°C | Lactiplantibacillus plantarum WCFS1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
for hydrolysis of lactose | Lactiplantibacillus plantarum WCFS1 |
7.5 | - |
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside | Lactiplantibacillus plantarum WCFS1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Lactiplantibacillus plantarum WCFS1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
lactose | at pH 7.0 and 60°C | Lactiplantibacillus plantarum WCFS1 | |
130 | - |
2-nitrophenyl beta-D-galactopyranoside | at pH 7.5 and 55°C | Lactiplantibacillus plantarum WCFS1 |