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Literature summary for 3.2.1.23 extracted from

  • Halbmayr, E.; Mathiesen, G.; Nguyen, T.H.; Maischberger, T.; Peterbauer, C.K.; Eijsink, V.G.; Haltrich, D.
    High-level expression of recombinant beta-galactosidases in Lactobacillus plantarum and Lactobacillus sakei using a Sakacin P-based expression system (2008), J. Agric. Food Chem., 56, 4710-4719.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of beta-galactosidase by expression of the genes encoding the large and the small subunit in Lactobacillus plantarum WCFS1. Cultivations yield about 23000 U of enzyme per l Limosilactobacillus reuteri

Cloned(Commentary)

Cloned (Comment) Organism
expression in Lactobacillus plantarum WCFS1 Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12
-
lactose recombinant enzyme, pH 6.5, 30°C Limosilactobacillus reuteri
13
-
lactose native enzyme, pH 6.5, 30°C Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri Q19R71 small subunit
-
Limosilactobacillus reuteri Q19R72 large subunit
-
Limosilactobacillus reuteri L103 Q19R71 small subunit
-
Limosilactobacillus reuteri L103 Q19R72 large subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactoside + H2O
-
Limosilactobacillus reuteri 2-nitrophenol + D-galactose
-
?
2-nitrophenyl beta-D-galactoside + H2O
-
Limosilactobacillus reuteri L103 2-nitrophenol + D-galactose
-
?
lactose + H2O
-
Limosilactobacillus reuteri D-galactose + D-glucose
-
?
lactose + H2O
-
Limosilactobacillus reuteri L103 D-galactose + D-glucose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
substrate 2-nitrophenyl-beta-D-galactoside Limosilactobacillus reuteri
55
-
substrate lactose Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 substrate lactose Limosilactobacillus reuteri
7
-
substrate 2-nitrophenyl-beta-D-galactoside Limosilactobacillus reuteri