Application | Comment | Organism |
---|---|---|
synthesis | production of beta-galactosidase by expression of the genes encoding the large and the small subunit in Lactobacillus plantarum WCFS1. Cultivations yield about 23000 U of enzyme per l | Limosilactobacillus reuteri |
Cloned (Comment) | Organism |
---|---|
expression in Lactobacillus plantarum WCFS1 | Limosilactobacillus reuteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
lactose | recombinant enzyme, pH 6.5, 30°C | Limosilactobacillus reuteri | |
13 | - |
lactose | native enzyme, pH 6.5, 30°C | Limosilactobacillus reuteri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | Q19R71 | small subunit | - |
Limosilactobacillus reuteri | Q19R72 | large subunit | - |
Limosilactobacillus reuteri L103 | Q19R71 | small subunit | - |
Limosilactobacillus reuteri L103 | Q19R72 | large subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri | 2-nitrophenol + D-galactose | - |
? | |
2-nitrophenyl beta-D-galactoside + H2O | - |
Limosilactobacillus reuteri L103 | 2-nitrophenol + D-galactose | - |
? | |
lactose + H2O | - |
Limosilactobacillus reuteri | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Limosilactobacillus reuteri L103 | D-galactose + D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
substrate 2-nitrophenyl-beta-D-galactoside | Limosilactobacillus reuteri |
55 | - |
substrate lactose | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | substrate lactose | Limosilactobacillus reuteri |
7 | - |
substrate 2-nitrophenyl-beta-D-galactoside | Limosilactobacillus reuteri |