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Literature summary for 3.2.1.23 extracted from

  • Hsu, C.; Yu, R.; Chou, C.
    Purification and characterization of a sodium-stimulated ?-galactosidasefrom Bifidobacterium longum CCRC 15708 (2006), World J. Microbiol. Biotechnol., 22, 355-361.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Bifidobacterium longum
Co2+
-
Bifidobacterium longum
Cu2+
-
Bifidobacterium longum
D-fructose
-
Bifidobacterium longum
Fe2+
-
Bifidobacterium longum
Fe3+
-
Bifidobacterium longum
lactose competitive Bifidobacterium longum
Mg2+
-
Bifidobacterium longum
Mn2+
-
Bifidobacterium longum
additional information poor inhibition by D-glucose, D-galactose, maltose, or raffinose Bifidobacterium longum
Zn2+
-
Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
2-nitrophenyl-beta-D-galactopyranoside pH 7.0, 50°C Bifidobacterium longum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10fold activation Bifidobacterium longum
Na+ 10fold activation Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
357000
-
native PAGE Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum
-
-
-
Bifidobacterium longum CCRC 15708
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 15.7fold by anion exchange chromatography and gel filtration Bifidobacterium longum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
168.6
-
purified enzyme Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Bifidobacterium longum 2-nitrophenol + beta-D-galactose
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Bifidobacterium longum CCRC 15708 2-nitrophenol + beta-D-galactose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bifidobacterium longum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
10 min, purified enzyme, stable up to Bifidobacterium longum
60
-
10 min, purified enzyme, inactivation Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bifidobacterium longum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7 purified enzyme, stable Bifidobacterium longum